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Invitrogen
XIAP Polyclonal Antibody
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FIGURE: 1 / 15
XIAP Antibody (PA5-29253) in ICC/IF
Immunofluorescence analysis of XIAP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-XIAP Rabbit Polyclonal Antibody (Product # PA5-29253) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
PA5-29253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human XIAP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, Mouse brain.
Predicted reactivity: Mouse (90%), Rat (88%), Dog (85%), Pig (89%), Rabbit (89%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Baculoviral IAP repeat-containing protein 4; BIR4; E3 ubiquitin-protein ligase XIAP; HILP; IAP homolog A; IAP-3; IAP-like protein; ILP; Inhibitor of apoptosis protein 3; RING-type E3 ubiquitin transferase XIAP; RP1-315G1.5; X-linked IAP; X-linked inhibitor of apoptosis protein; X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
Gene Aliases: 1110015C02Rik; Aipa; API3; BIRC4; hIAP-3; hIAP3; IAP-3; IAP3; ILP-1; ILP1; MIHA; XIAP; XLP2
UniProt ID: (Human) P98170, (Mouse) Q60989
Entrez Gene ID: (Human) 331, (Mouse) 11798
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
identical protein binding
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
ubiquitin protein ligase activity
protease binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
scaffold protein binding
protease inhibitor
apoptotic process
cellular response to DNA damage stimulus
Wnt signaling pathway
protein ubiquitination
regulation of BMP signaling pathway
positive regulation of protein ubiquitination
regulation of cell proliferation
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of innate immune response
regulation of inflammatory response
copper ion homeostasis
regulation of nucleotide-binding oligomerization domain containing signaling pathway
positive regulation of canonical Wnt signaling pathway
mitotic spindle assembly
positive regulation of protein linear polyubiquitination
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of neuron apoptotic process
neuron apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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