Invitrogen

XPA Polyclonal Antibody

View all (17) XPA antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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XPA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human prostate carcinoma tissue using XPA Polyclonal Antibody (Product # PA5-119824). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the XPA antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

XPA Antibody in Western Blot (WB) — Western blot analysis of XPA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. XPA Polyclonal Antibody (Product # PA5-119824) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate. Lane 2: A431 cell lysate.

Product Details

PA5-119824

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within human XPA aa 20-60

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2913396

Product Specific Information

Positive Control: Hela cell lysate, A431 cell lysate, rat large intestine tissue, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human prostate carcinoma tissue, human breast tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, mouse testis tissue, mouse kidney tissue.

Subcellular Location: Nucleus.

Target Information

The XPA (xeroderma pigmentosumgroup A) protein specifically recognizes the UV-orchemically damaged DNA lesions, and triggers thenucleotide excision repair process. XPA binds to thereplication protein A (RPA) or the excision repaircross complementing 1 protein (ERCC 1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a >2000 fold increased risk to develop skin cancer atsun-exposed areas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA repair protein complementing XP-A cells; DNA repair protein complementing XP-A cells homolog; excision repair-controlling; mutant xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A-complementing protein; Xeroderma pigmentosum group A-complementing protein homolog; xeroderma pigmentosum, complementation group A

Gene Aliases: AI573865; XP1; XPA; XPAC

UniProt ID: (Human) P23025, (Mouse) Q64267

Entrez Gene ID: (Human) 7507, (Mouse) 22590, (Rat) 298074

Molecular Function: damaged DNA-binding protein