Immunofluorescent analysis of XPC (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a XPC polyclonal antibody (Product # PA3-956) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Two synthetic peptides, individually conjugated to the C-Terminus and N-Terminus respectively of human XPC.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-956 detects XPC in human and mouse cells.
PA3-956 has been successfully used in Western blotting and ICC/IF procedures. By Western blot it detects a ~125 kDa protein representing XPC.
The PA3-956 immunogen is two synthetic peptides, individually conjugated to the C-Terminus and N-Terminus respectively of human XPC.
XPC is a damage recognition/structure distortion factor used in nucleotide excision repair (NER). XPC exhibits the strongest affinity for damaged DNA, and showed a remarkable preference to UV-damaged DNA. It has been suggested as the initiator of global genome NER, and a recent immunohistochemistry study also strongly supports a role for XPC as a global initiator in repair. XPC is also involved in the recruitment on TFIIH to damaged DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.