Western blot analysis of Jurkat Whole Cell Lysate using PA5-19757, XPD primary antibody at a dilution of 2 ug/ml (lane 1). PA5-19757 staining of Ramos Whole Cell Lysate at a dilution of 2 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human XPD.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||2 µg/ml|
This antibody is predicted to react with mouse, cow and pig based on sequence homology.
The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
basic transcription factor 2 80 kDa subunit; BTF2 p80; COFS2, EM9, MGC102762, MGC126218, MGC126219, TTD, XPD, BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; excision repair cross-complementation group 2; excision repair cross-complementing rodent repair deficiency, complementation group 2; TFIIH 80 kDa subunit; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH basal transcription factor complex helicase subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p80; xeroderma pigmentosum complementary group D; xeroderma pigmentosum group D-complementing pr; xeroderma pigmentosum group D-complementing protein
COFS2; EM9; ERCC2; TFIIH; TTD; TTD1; XPD; XPDC