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Invitrogen
XPF Polyclonal Antibody
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FIGURE: 1 / 2
XPF Antibody (PA5-117118) in WB
Western blot analysis of XPF in extracts of various cell lines. Samples were incubated with XPF Polyclonal antibody (Product # PA5-117118) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-117118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Form
Liquid
Concentration
1.49 mg/mL
Amount
149 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-251MG, HT-1080
Immunogen sequence: GYRREISSSP ESCPEEIKHE EFDVNLSSDA AFGILKEPLT IIHPLLGCSD PYALTRVLHE VEPRYVVLYD AELTFVRQLE IYRASRPGKP LRVYFLIYGG STEEQRYLTA LRKEKEAFEK LIREKASMVV PEEREGRDET NLDLVRGTAS ADVSTDTRKA GGQEQNGTQQ SIVVDMREFR SELPSLIHRR GIDIEPVTLE VGDYILTPEM CVERKSISDL IGSLNNGRLY SQCISMSRYY KRPVLLIEFD PSKPFSLTSR GALFQEISSN
Target Information
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA excision repair protein ERCC-4; DNA repair endonuclease subunit; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; Ercc4 protein-like; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; unnamed protein product; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
Gene Aliases: AI606920; ERCC11; ERCC4; FANCQ; RAD1; RGD1560340; XFEPS; XPF
UniProt ID: (Human) Q92889, (Mouse) Q9QZD4
Entrez Gene ID: (Human) 2072, (Mouse) 50505, (Rat) 304719
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
DNA binding
damaged DNA binding
single-stranded DNA binding
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
protein binding
telomerase inhibitor activity
hydrolase activity
identical protein binding
3' overhang single-stranded DNA endodeoxyribonuclease activity
promoter-specific chromatin binding
nucleic acid binding
protein C-terminus binding
phosphoric ester hydrolase activity
structure-specific DNA binding
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
protein N-terminus binding
DNA metabolism protein
endodeoxyribonuclease
nucleic acid metabolism protein
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA repair
nucleotide-excision repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
response to UV
UV protection
regulation of autophagy
negative regulation of telomere maintenance
cellular response to UV
telomeric DNA-containing double minutes formation
nucleotide-excision repair involved in interstrand cross-link repair
negative regulation of telomere maintenance via telomere lengthening
negative regulation of protection from non-homologous end joining at telomere
DNA metabolic process
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA incision
DNA catabolic process, endonucleolytic
obsolete telomere maintenance via telomere shortening
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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