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Invitrogen
XPF Polyclonal Antibody
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FIGURE: 1 / 1
XPF Antibody (PA5-77973) in WB
Western blot analysis of XPF in various lysates. Samples were incubated with XPF polyclonal antibody (Product # PA5-77973) using a dilution of 1:10,000. A. 30 μg 293T whole cell extract; B. 30 μg A431 whole cell extract; C. 30 μg HeLa whole cell extract; D. 30 μg HepG2 whole cell extract. Separated by 5 % SDS-PAGE.
Product Details
PA5-77973
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human XPF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
2.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Mouse (84%), Chicken (83%), Bovine (88%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA excision repair protein ERCC-4; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; unnamed protein product; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
Gene Aliases: ERCC11; ERCC4; FANCQ; RAD1; XFEPS; XPF
UniProt ID: (Human) Q92889
Entrez Gene ID: (Human) 2072
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
DNA binding
damaged DNA binding
single-stranded DNA binding
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
protein binding
telomerase inhibitor activity
hydrolase activity
identical protein binding
3' overhang single-stranded DNA endodeoxyribonuclease activity
promoter-specific chromatin binding
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA repair
nucleotide-excision repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
response to UV
UV protection
regulation of autophagy
negative regulation of telomere maintenance
cellular response to UV
telomeric DNA-containing double minutes formation
nucleotide-excision repair involved in interstrand cross-link repair
negative regulation of telomere maintenance via telomere lengthening
negative regulation of protection from non-homologous end joining at telomere
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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