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Product Details
UM500022
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
UMAB22
Immunogen
Full length human recombinant protein of human XPF produced in HEK293 cell.
Conjugate
Unconjugated
Form
liquid
Concentration
0.5-1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA excision repair protein ERCC-4; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F
Gene Aliases: ERCC11; ERCC4; FANCQ; RAD1; XFEPS; XPF
UniProt ID: (Human) Q92889
Entrez Gene ID: (Human) 2072
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
damaged DNA binding
single-stranded DNA binding
endodeoxyribonuclease activity
protein binding
protein C-terminus binding
structure-specific DNA binding
protein N-terminus binding
DNA metabolism protein
endodeoxyribonuclease
nucleic acid metabolism protein
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
response to UV
UV protection
negative regulation of telomere maintenance
nucleotide-excision repair, DNA incision
cellular response to UV
interstrand cross-link repair
global genome nucleotide-excision repair
nucleotide-excision repair involved in interstrand cross-link repair
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