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XRCC1 Polyclonal Antibody

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XRCC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 21468-1-AP (XRCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

XRCC1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

21468-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XRCC1 Fusion Protein Ag15172 (284-633 aa encoded by BC023593) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: TAPVPARAQ GAVTGKPRGE GTEPRRPRAG PEELGKILQG VVVVLSGFQN PFRSELRDKA LELGAKYRPD WTRDSTHLIC AFANTPKYSQ VLGLGGRIVR KEWVLDCHRM RRRLPSQRYL MAGPGSSSEE DEASHSGGSG DEAPKLPQKQ PQTKTKPTQA AGPSSPQKPP TPEETKAASP VLQEDIDIEG VQSEGQDNGA EDSGDTEDEL RRVAEQKEHR LPPGQEENGE DPYAGSTDEN TDSEEHQEPP DLPVPELPDF FQGKHFFLYG EFPGDERRKL IRYVTAFNGE LEDYMSDRVQ FVITAQEWDP SFEEALMDNP SLAFVRPRWI YSCNEKQKLL PHQLYGVVPQ A (284-633 aa encoded by BC023593)

Target Information

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomes Serve as Nanoparticles to Deliver Anti-miR-214 to Reverse Chemoresistance to Cisplatin in Gastric Cancer.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang X,Zhang H,Bai M,Ning T,Ge S,Deng T,Liu R,Zhang L,Ying G,Ba Y

Wed Mar 07 00:00:00 EST 2018

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: DNA repair protein XRCC1; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1

Gene Aliases: RCC; Xrcc-1; XRCC1

UniProt ID: (Human) P18887, (Mouse) Q60596

Entrez Gene ID: (Human) 7515, (Mouse) 22594

Molecular Function: DNA metabolism protein nucleic acid metabolism protein