Invitrogen

XRCC1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (42) XRCC1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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XRCC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of XRCC1 in U-251 MG cells using a XRCC1 polyclonal antibody (Product # PA5-82259). The analysis shows localization to nucleoplasm.

XRCC1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of XRCC1 in human pancreas using a XRCC1 polyclonal antibody (Product # PA5-82259). The analysis shows moderate to strong nuclear positivity in exocrine glandular cells.

XRCC1 Antibody in Western Blot (WB) — Western blot analysis of XRCC1 by a XRCC1 polyclonal antibody (Product # PA5-82259). Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC1.

XRCC1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-549 cells were transfected with, control siRNA, XRCC1 siRNA probe #1, and XRCC1 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-XRCC1 Polyclonal Antibody (Product # PA5-82259) used at a dilution of 0.4 µg/mL. Anti-XRCC1 was used as a loading control. {KD}

Product Details

PA5-82259

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human XRCC1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Anitgen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2789419

Product Specific Information

Immunogen sequence: WDRVKIVCSQ PYSKDSPFGL SFVRFHSPPD KDEAEAPSQK VTVTKLGQFR VKEEDESANS LRPGALFFSR INKTSPVTAS DPAGPSYAAA TLQASSAASS ASPVSRAIGS TSKPQESPKG KRKLDLNQEE KKT

Target Information

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA repair protein XRCC1; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1

Gene Aliases: RCC; XRCC1

UniProt ID: (Human) P18887

Entrez Gene ID: (Human) 7515

Molecular Function: DNA metabolism protein

Function(s)

single strand break repair double-strand break repair via homologous recombination response to hypoxia transcription-coupled nucleotide-excision repair base-excision repair base-excision repair, DNA ligation nucleotide-excision repair, DNA gap filling response to organic substance response to drug

Process(es)

damaged DNA binding DNA ligase activity protein binding enzyme binding

XRCC1 Polyclonal Antibody

Product Details

PA5-82259

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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