Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is testis tissue.
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad5. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair.
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Protein Aliases: DKFZp781P0919; DNA repair protein XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; X-ray repair cross-complementing protein 2
Gene Aliases: FANCU; XRCC2
UniProt ID: (Human) O43543
Entrez Gene ID: (Human) 7516