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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic partial peptide of human XRCC3.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for XRCC3; no cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D.
This antibody is not recommended for ICC/IF applications.
Suggested positive control: antigen standard for XRCC3 (transient overexpression lysate), TK6 lysates and 100 ug HeLa lysates.
Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homolgs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
X ray repair complementing defective repair in Chinese hamster cells 3; X ray repair cross complementing protein 3; X-ray repair complementing defective repair in Chinese hamster cells 3; X-ray repair cross-complementing protein 3