Western blot analysis of XRCC3 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of DDDDK-human XRCC3-transfected 293T cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with a XRCC3 polyclonal antibody (Product # PA5-27103) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 158 of Human XRCC3|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27103 targets XRCC3 in WB applications and shows reactivity with Human samples.
The PA5-27103 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 158 of Human XRCC3.
This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified.
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