Proteintech

XRCC4 Polyclonal Antibody

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XRCC4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

XRCC4 Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

XRCC4 Antibody in Immunoprecipitation (IP) — IP result of anti-XRCC4 (IP:15817-1-AP, 4ug; Detection:15817-1-AP 1:800) with HepG2 cells lysate 3200ug.

Product Details

15817-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XRCC4 Fusion Protein Ag8606 (6-310 aa encoded by BC005259) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.28 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: SRIHLVSEP SITHFLQVSW EKTLESGFVI TLTDGHSAWT GTVSESEISQ EADDMAMEKG KYVGELRKAL LSGAGPADVY TFNFSKESCY FFFEKNLKDV SFRLGSFNLE KVENPAEVIR ELICYCLDTI AENQAKNEHL QKENERLLRD WNDVQGRFEK CVSAKEALET DLYKRFILVL NEKKTKIRSL HNKLLNAAQE REKDIKQEGE TAICSEMTAD RDPVYDESTD EESENQTDLS GLASAAVSKD DSIISSLDVT DIAPSRKRRQ RMQRNLGTEP KMAPQENQLQ EKEKGRKKET SEKEAV (6-310 aa encoded by B C005259)

Target Information

The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

PloS one

Jiang W,Li YQ,Liu N,Sun Y,He QM,Jiang N,Xu YF,Chen L,Ma J

Thu Dec 17 00:00:00 EST 2015

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Toxicology and applied pharmacology

Dong H,Shi Q,Song X,Fu J,Hu L,Xu D,Su C,Xia X,Song E,Song Y

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: DNA repair protein XRCC4; hXRCC4; Protein XRCC4, C-terminus; X-ray repair complementing defective repair in Chinese hamster cells 4; X-ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; XRCC4/C

Gene Aliases: SSMED; XRCC4

UniProt ID: (Human) Q13426

Entrez Gene ID: (Human) 7518, (Rat) 309995

Molecular Function: DNA metabolism protein nucleic acid metabolism protein