|Western Blot (WB)||1:200-1:1000|
|Tested Species reactivity||Human, Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: IAPSRKRRQRMQRN conjugated to BSA, corresponding to amino acids 266-279 of Human XRCC4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% ammonium sulfate|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-25419 detects XRCC4 from human, mouse, and rat samples.
PA1-25419 has been successfully used in Western blot and IHC procedures.
The PA1-25419 immunogen is a synthetic peptide, conjugated to BSA, corresponding to residues I(226) A P S R K R R Q R M Q R N(279) of human XRCC4.
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants.
Protein Aliases: DNA repair protein XRCC4; X-ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; X-ray repair, complementing defective, repair in Chinese hamster
Gene Aliases: 2310057B22Rik; AW413319; AW545101
Molecular Function: Nucleic acid binding