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|Tested species reactivity||Human, Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: IAPSRKRRQRMQRN conjugated to BSA, corresponding to amino acids 266-279 of Human XRCC4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% ammonium sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25419 detects XRCC4 from human, mouse, and rat samples.
PA1-25419 has been successfully used in Western blot and IHC procedures.
The PA1-25419 immunogen is a synthetic peptide, conjugated to BSA, corresponding to residues I(226) A P S R K R R Q R M Q R N(279) of human XRCC4.
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA repair protein XRCC4; X-ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; X-ray repair, complementing defective, repair in Chinese hamster
2310057B22Rik; AW413319; AW545101