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|Tested species reactivity||Tag|
|Published species reactivity||Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Xpress synthetic peptide Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
R910-25 is designed to specifically interact with the Xpress™ leader peptide (-Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys-).
R910-25 is tested in Western blot against 100ng of an E. coli expressed fusion protein containing the Xpress epitope. This product contains enough material for 25 Western blots.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Epitope tag detection with an unconjugated antibody requires the use of a secondary antibody that carries a reporter for detection. Unconjugated antibodies let you choose the detection method which best suits your needs. They are also useful for the detection of weak signals, since a secondary antibody can amplify the signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Dynactin integrity depends upon direct binding of dynamitin to Arp1.
R910-25 was used in western blot to study the binding of dynamitin to Arp1 and its role in dynactin integrity.
|Cheong FK,Feng L,Sarkeshik A,Yates JR,Schroer TA||Molecular biology of the cell (25:2171)||2014|