|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 32 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunoprecipitation (IP)||See 5 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Tag|
|Published species reactivity||Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Xpress synthetic peptide Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C|
R910-25 is designed to specifically interact with the Xpress™ leader peptide (-Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys-).
R910-25 is tested in Western blot against 100ng of an E. coli expressed fusion protein containing the Xpress epitope. This product contains enough material for 25 Western blots.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Epitope tag detection with an unconjugated antibody requires the use of a secondary antibody that carries a reporter for detection. Unconjugated antibodies let you choose the detection method which best suits your needs. They are also useful for the detection of weak signals, since a secondary antibody can amplify the signal. Xpress sequence: DLYDDDDK (Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DLYDDDDK; Xpress Tag
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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