Invitrogen

YAP1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

13 References

View all (57) YAP1 antibodies

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YAP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of YAP1 was performed using 70% confluent log phase HeLa cells treated with serum starvation for 12 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with YAP1 Polyclonal Antibody (Product # PA1-46189) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows untreated cells with predominant nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

YAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of YAP1 in immersion fixed paraffin-embedded sections of human placenta. Samples were incubated in YAP1 polyclonal antibody (Product # PA1-46189) using a dilution of 1:200 for 1 hour at room temperature followed by the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.

YAP1 Antibody in Western Blot (WB) — Knockout of YAP1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR772157_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of YAP1 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa YAP1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-YAP1 Polyclonal Antibody (Product # PA1-46189, 1:2,000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:8,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to YAP1.

YAP1 Antibody in ChIP Assay (ChIP) — Enrichment of endogenous YAP1 protein at specific gene loci using Anti-YAP1 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-YAP1 Rabbit Polyclonal Antibody (Product # PA1-46189, 3 ug) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP System (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over CYR61, CTGF, ANKRD1 (active) and SAT alpha (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

YAP1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 4 Fbxw7 regulates the stability of the YAP protein in HCC cells. A) HepG2 and Hep3B cells that were transfected with Flag-Fbxw7 and Fbxw7-shRNA, respectively, and subjected to Western blotting for Fbxw7, c-Myc, Cyclin E and YAP. Fbxw7 overexpression decreased c-Myc, Cyclin E and YAP protein levels in HepG2 cells, whereas Fbxw7 knockdown led to c-Myc, Cyclin E and YAP accumulation in Hep3B cells. Data are representative of multiple repeats with similar results. B) HepG2 cells transfected with EV or Flag-Fbxw7 and Hep3B cells transfected with non-targeting (NT)-shRNA or Fbxw7-shRNA were harvested for RNA extraction and real time RT-PCR. Fbxw7 overexpression or knockdown did not change YAP mRNA levels. n = 3 independent experiment; Values are depicted as the Mean +- SEM. C) HCC cells that were treated as above were subjected to IF for YAP. Quantification of YAP immunofluorescence revealed that the average level of YAP in the control cells was significantly higher than that in the Fbxw7 overexpressing HepG2 cells and lower than that in the Fbxw7 shRNA transfected Hep3B cells. Scale bar: 20 mum, n = 6; Values are depicted as the Mean +- SEM; ** P < 0.01 by t test.

YAP1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Figure 3 Immunohistochemical analyses of YAP and its correlation with Fbxw7 protein in HCC. In cases of high Fbxw7 protein expression (D, F) , there was no detectable YAP protein expression (A, C) in the same tissue section. In contrast, in the case of low Fbxw7 protein expression (E) , there was strong YAP protein expression (B) . The fibrotic septa (B, E) simply represent fibrotic tumor stroma with normal background liver. Scale bar: 100 mum.

YAP1 Antibody in Immunohistochemistry (IHC) — Fig. 7 AOS inhibits the prostate tumor formation in nude mice through the Hippo/YAP pathway. a Morphological images of tumors that were engrafted and AOS treated. b Solid tumors were isolated from nude mice. c , d Average tumor weights ( c ) and sizes ( d ) were measured in different groups. e , f Western blot assay ( e ) and IHC ( f ) were used to detect the expression levels of the main signaling molecules of the Hippo/YAP in tumor tissues. * P < 0.05

YAP1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inYAP1 KO cell line compared to control cell line using Anti-YAP1 Polyclonal Antibody (Product # PA1-46189). {KO}

Product Details

PA1-46189

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1:500	View 3 publications 3 publications
Immunoprecipitation (IP)	2-10 µg	-
ChIP assay (ChIP)	1.5 µg/10^6 cells	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Dog, Human, Mouse, Rat, Zebrafish
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant YAP1 protein expressed in bacteria.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2219137

Product Specific Information

Suggested positive control: brain and kidney lysates, and HEK 293 cell lysate, 293 whole cell lysate, antigen standard for YAP1 (transient overexpression lysate), human kidney protein, human brain protein.

Target Information

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation.

PloS one

Geiger F,Schnitzler LG,Brugger MS,Westerhausen C,Engelke H

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunocytochemistry

Tue Apr 26 00:00:00 EDT 2022

Directed invasion of cancer cell spheroids inside 3D collagen matrices oriented by microfluidic flow in experiment and simulation.

PloS one

Geiger F,Schnitzler LG,Brugger MS,Westerhausen C,Engelke H

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunocytochemistry

Tue Apr 26 00:00:00 EDT 2022

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation.

Cell death and differentiation

Canu V,Donzelli S,Sacconi A,Lo Sardo F,Pulito C,Bossel N,Di Benedetto A,Muti P,Botti C,Domany E,Bicciato S,Strano S,Yarden Y,Blandino G

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in ChIP assay

Sat May 01 00:00:00 EDT 2021

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation.

Cell death and differentiation

Canu V,Donzelli S,Sacconi A,Lo Sardo F,Pulito C,Bossel N,Di Benedetto A,Muti P,Botti C,Domany E,Bicciato S,Strano S,Yarden Y,Blandino G

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in ChIP assay

Sat May 01 00:00:00 EDT 2021

Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.

The Journal of international medical research

Li H,Kan B,Song L,Liu Y,Jian X

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Tue Sep 01 00:00:00 EDT 2020

Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis.

The Journal of international medical research

Li H,Kan B,Song L,Liu Y,Jian X

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Western Blot

Tue Sep 01 00:00:00 EDT 2020

PI3K Inhibitors Curtail MYC-Dependent Mutant p53 Gain-of-Function in Head and Neck Squamous Cell Carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ganci F,Pulito C,Valsoni S,Sacconi A,Turco C,Vahabi M,Manciocco V,Mazza EMC,Meens J,Karamboulas C,Nichols AC,Covello R,Pellini R,Spriano G,Sanguineti G,Muti P,Bicciato S,Ailles L,Strano S,Fontemaggi G,Blandino G

PA1-46189 was used in ChIP assay to investigate mutant p53-associated functions through gene set enrichment analysis in the Cancer Genome Atlas cohort of head and neck squamous cell carcinoma and in a panel of 22 head and neck squamous cell carcinoma cell lines.

Mon Jun 15 00:00:00 EDT 2020

PI3K Inhibitors Curtail MYC-Dependent Mutant p53 Gain-of-Function in Head and Neck Squamous Cell Carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Mon Jun 15 00:00:00 EDT 2020

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Cell death & disease

Han Y,Zhang L,Yu X,Wang S,Xu C,Yin H,Wang S

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunohistochemistry

Fri May 10 00:00:00 EDT 2019

NSCLC depend upon YAP expression and nuclear localization after acquiring resistance to EGFR inhibitors.

Genes & cancer

McGowan M,Kleinberg L,Halvorsen AR,Helland Å,Brustugun OT

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Wed Mar 01 00:00:00 EST 2017

The clinical significance of forkhead box protein A1 and its role in colorectal cancer.

Molecular medicine reports

Ma W,Jiang J,Li M,Wang H,Zhang H,He X,Huang L,Zhou Q

PA1-46189 was used in western blot examine the impact of FOXA1 to colorectal cancer

Thu Sep 01 00:00:00 EDT 2016

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Sat May 17 00:00:00 EDT 2014

Fbxw7 is an independent prognostic marker and induces apoptosis and growth arrest by regulating YAP abundance in hepatocellular carcinoma.

Molecular cancer

Tu K,Yang W,Li C,Zheng X,Lu Z,Guo C,Yao Y,Liu Q

Published figure using YAP1 polyclonal antibody (Product # PA1-46189) in Immunofluorescence

Sat May 17 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; yap; Yes associated protein 1; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog

Gene Aliases: AI325207; cb194; CH211-181P1.5-001; COB1; sb:cb194; si:ch211-181p1.5; si:dkey-3b8.3; wu:fc18c04; YAP; YAP1; YAP2; YAP65; YKI; Yorkie; zgc:158380; zYAP

UniProt ID: (Human) P46937, (Rat) Q2EJA0, (Mouse) P46938, (Zebrafish) Q1L8J7

Entrez Gene ID: (Dog) 479465, (Human) 10413, (Rat) 363014, (Mouse) 22601, (Zebrafish) 561411

Molecular Function: transcription cofactor

Function(s)

cell morphogenesis vasculogenesis embryonic heart tube morphogenesis transcription initiation from RNA polymerase II promoter cellular response to DNA damage stimulus cell proliferation positive regulation of cell proliferation regulation of keratinocyte proliferation keratinocyte differentiation negative regulation of epithelial cell differentiation notochord development somatic stem cell population maintenance hippo signaling positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth paraxial mesoderm development lateral mesoderm development regulation of neurogenesis contact inhibition bud elongation involved in lung branching lung epithelial cell differentiation cellular response to retinoic acid cellular response to gamma radiation regulation of stem cell proliferation regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of canonical Wnt signaling pathway positive regulation of stem cell population maintenance negative regulation of nucleic acid-templated transcription negative regulation of stem cell differentiation negative regulation of extrinsic apoptotic signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter DNA damage induced protein phosphorylation regulation of cell proliferation regulation of canonical Wnt signaling pathway liver development secondary heart field specification ventriculo bulbo valve development retinal pigment epithelium development brain development endoderm development dorsal/ventral axis specification negative regulation of gene expression BMP signaling pathway pancreas development fin regeneration embryonic hemopoiesis pronephric duct development glucose homeostasis negative regulation of apoptotic process regulation of organ growth blood vessel morphogenesis posterior lateral line development cartilage development cilium morphogenesis heart formation positive regulation of glutamate-ammonia ligase activity

Process(es)

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding chromatin binding transcription coactivator activity transcription corepressor activity protein binding protein C-terminus binding transcription regulatory region DNA binding proline-rich region binding

YAP1 Polyclonal Antibody

Product Details

PA1-46189

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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