Invitrogen

YARS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (12) YARS antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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YARS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of YARS in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a YARS Polyclonal Antibody (Product # PA5-53883).

YARS Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of YARS in human cerebral cortex, kidney, liver and pancreas using YARS Polyclonal Antibody (Product # PA5-53883) (A) shows similar protein distribution across tissues to an independent YARS Polyclonal Antibody (B).

YARS Antibody in Western Blot (WB) — Western blot analysis of YARS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a YARS Polyclonal Antibody (Product # PA5-53883).

YARS Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U2OS cells were transfected with, control siRNA, YARS siRNA probe #1, and YARS siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-YARS Polyclonal Antibody (Product # PA5-53883) used at a dilution of 0.4 µg/mL. {KD}

Product Details

PA5-53883

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human YARS View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2649715

Product Specific Information

Immunogen sequence: GNVENNGVLS FIKHVLFPLK SEFVILRDEK WGGNKTYTAY VDLEKDFAAE VVHPGDLKNS VEVALNKLLD PIREKFNTPA LKKLASAAYP DPSKQKPMAK GPAKNSEPEE VIPSRLDIRV GKIITVEKHP DAD

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 93%.

Target Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: tyrosine tRNA ligase 1, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed; tyrosyl trna synthetase; tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; TyrRS

Gene Aliases: AL024047; AU018965; CMTDIC; TYRRS; YARS; YARS1; YRS; YTS

UniProt ID: (Human) P54577, (Mouse) Q91WQ3, (Rat) Q4KM49

Entrez Gene ID: (Human) 8565, (Mouse) 107271, (Rat) 313047

Molecular Function: translational protein