Invitrogen

YARS Polyclonal Antibody

Product Image Gallery

YARS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of YARS in SH-SY5Y cells using a YARS polyclonal antibody (Product # PA5-97547) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

YARS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of YARS in paraffin embedded human testis tissue using a YARS polyclonal antibody (Product # PA5-97547) at a dilution of 1:100.

YARS Antibody in Western Blot (WB) — Western Blot analysis of YARS using a YARS Polyclonal antibody (Product # PA5-97547) at a concentration of 2 µg/mL. Positive WB detected in 293T whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:15,000 dilution. Observed band size: 60 kDa.

Product Details

PA5-97547

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Tyrosine--tRNA ligase, cytoplasmic protein (2-528AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	3 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812163

Target Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: tyrosine tRNA ligase 1, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic; Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed; tyrosyl trna synthetase; tyrosyl--tRNA ligase; Tyrosyl-tRNA synthetase; tyrosyl-tRNA synthetase, cytoplasmic; TyrRS

Gene Aliases: CMTDIC; TYRRS; YARS; YARS1; YRS; YTS

UniProt ID: (Human) P54577

Entrez Gene ID: (Human) 8565

Molecular Function: translational protein