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Invitrogen
YTHDC2 Polyclonal Antibody
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FIGURE: 1 / 4
YTHDC2 Antibody (PA5-144005) in ICC/IF
Immunocytochemistry analysis of YTHDC2 in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in YTHDC2 Polyclonal antibody (Product # PA5-144005) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate... View More
Product Details
PA5-144005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human YTHDC2 recombinant protein (Position: K105-K1231).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Daudi whole cell, human Hela whole cell, human 293T whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. ICC/IF: SiHa cell. Flow: MCF-7 cell, SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' RNA helicase YTHDC2; CsA-associated helicase-like protein; DKFZp564A186; FLJ10053; FLJ2194; hYTHDC2; Keen to exit meiosis leaving testes under-populated protein; Ketu; mYTHDC2; probable ATP-dependent RNA helicase YTHDC2; unnamed protein product; YTH domain containing 2; YTH domain containing 2-like; YTH domain-containing protein 2; YTH domain-containing protein C2
Gene Aliases: 3010002F02Rik; BC037178; CAHL; hYTHDC2; RGD1563192; YTHDC2
UniProt ID: (Human) Q9H6S0, (Mouse) B2RR83
Entrez Gene ID: (Human) 64848, (Rat) 307446, (Mouse) 240255
nucleotide binding
nucleic acid binding
RNA binding
RNA helicase activity
helicase activity
protein binding
ATP binding
RNA-dependent ATPase activity
hydrolase activity
ATPase activity
3'-5' RNA helicase activity
RNA polymerase binding
N6-methyladenosine-containing RNA binding
ATP-dependent RNA helicase activity
poly(A) RNA binding
RNA helicase
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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