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Zeta
ER Recombinant Rabbit Monoclonal Antibody (ZR147), RAbMono™
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Product Details
Z2691RS
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
CHO cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ZR147
Immunogen
Recombinant fragment (around aa495-595) of human ER alpha protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
tris with BSA, NP-40
Contains
<0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
This product is diluted and in a ready-to-use formulation.
A recommended positive control tissue for this product is Breast Carcinoma, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686N23123; E2 receptor alpha; ER; ER delta E7 isoform; ER-alpha; ER-alpha; del.3*,+1*,4,5,6,7* variant; ER-alpha; del.3*,4,5,6,7*/770 variant; ER-alpha; del.3*,4,5,6,7,8*/862 variant; ER-alpha; del.4*,5*,7 variant; ER-alpha; del.4*,5,6*/548 variant; ER-alpha; del.4*,5,6,7*/714 variant; ER-alpha; del.4*,5,6,7,8*/754 variant; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/819 cingulate gyrus isoform; ER-alpha; estrogen receptor alpha delta 3*,4,5,6,7*/833 superior frontal gyrus isoform; ER-alpha; estrogen receptor alpha delta 4*,5,6,7*/747 substantia nigra isoform; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor-related protein; hER-alpha36; lacks a part of the exon 3, exons 4-6, and part of exon 7; contains an 822 base in frame deletion; lacks most of the exon 3, exons 4-7, and part of exon 8; lacks most of the exon 4, exons 5-6, and part of exon 7; lacks the end of the exon 3, exons 4-7, and part of exon 8; MB1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha; receptor; RP1-130E4.1; steroid hormone receptor; unnamed protein product
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
Entrez Gene ID: (Human) 2099
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
TFIIB-class transcription factor binding
transcription cofactor binding
transcription corepressor binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
calmodulin binding
beta-catenin binding
zinc ion binding
lipid binding
TBP-class protein binding
enzyme binding
protein kinase binding
nitric-oxide synthase regulator activity
estrogen receptor activity
estrogen receptor binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
metal ion binding
ATPase binding
14-3-3 protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
antral ovarian follicle growth
epithelial cell development
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
androgen metabolic process
male gonad development
negative regulation of gene expression
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
response to estradiol
regulation of toll-like receptor signaling pathway
negative regulation of smooth muscle cell apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
response to estrogen
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
fibroblast proliferation
positive regulation of fibroblast proliferation
stem cell differentiation
regulation of inflammatory response
positive regulation of nitric-oxide synthase activity
positive regulation of sequence-specific DNA binding transcription factor activity
RNA polymerase II transcriptional preinitiation complex assembly
uterus development
vagina development
prostate epithelial cord elongation
prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
regulation of branching involved in prostate gland morphogenesis
mammary gland branching involved in pregnancy
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
protein localization to chromatin
cellular response to estrogen stimulus
cellular response to estradiol stimulus
cellular response to oxygen-containing compound
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of epithelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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