Invitrogen

ZACN Polyclonal Antibody

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ZACN Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of ZACN in SiHa cells using a ZACN polyclonal antibody (Product #PA5-114309). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

ZACN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ZACN in paraffin-embedded mouse cerebellum tissue using a polyclonal antibody (Product #PA5-114309). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ZACN Antibody in Western Blot (WB) — Western blot analysis of ZACN using a ZACN polyclonal antibody (Product #PA5-114309). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human kidney tissue lysate, Lane 2: HepG2 cell lysate

ZACN Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of ZACN using SH-SY5Y cells and a ZACN polyclonal antibody (Product #PA5-114309). The cells were fixed, permeabilized and stained with the primary antibody at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

PA5-114309

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
Flow Cytometry (Flow)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide within Human ZAC aa 139-188 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

ZACN is a zinc-activated ligand-gated ion channel that defines a new subgroup of the cysteine-loop superfamily of ligand-gated ion channels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ligand-gated ion channel subunit; Ligand-gated ion channel zinc-activated 1; ligand-gated ion channel, zinc activated 1; Ligand-gated ion-channel receptor L2; zinc activated ligand-gated ion channel 1; Zinc-activated ligand-gated ion channel

Gene Aliases: L2; LGICZ; LGICZ1; ZAC; ZAC1; ZACN

UniProt ID: (Human) Q401N2

Entrez Gene ID: (Human) 353174

Molecular Function: ligand-gated ion channel