Bethyl Laboratories

ZAK Polyclonal Antibody

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ZAK Antibody in Western Blot (WB) — **Detection of human ZAK by western blot and immunoprecipitation.** *Samples:* Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. *Antibodies:* Affinity purified rabbit anti-ZAK antibody A301-993A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. ZAK was also immunoprecipitated by rabbit anti-ZAK antibody A301-994A, which recognizes a downstream epitope. *Detection:* Chemiluminescence with exposure times of 30 seconds (A and B).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 700 and 750

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif. This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HCCS-4; Human cervical cancer suppressor gene 4 protein; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen-activated protein kinase kinase kinase 20; Mitogen-activated protein kinase kinase kinase MLT; mixed lineage kinase 7; mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; mixed lineage kinase-related kinase MRK-beta; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; Sterile alpha motif- and leucine zipper-containing kinase AZK

Gene Aliases: AZK; HCCS4; MAP3K20; MLK7; mlklak; MLT; MLTK; MRK; pk; SFMMP; ZAK

UniProt ID: (Human) Q9NYL2

Entrez Gene ID: (Human) 51776

Molecular Function: kinase non-receptor serine/threonine protein kinase non-receptor tyrosine protein kinase protein kinase transferase