Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PA5-21084
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1 µg/mL | - |
Immunohistochemistry (IHC) |
2.5 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
An 18 amino acid peptide near the carboxy terminus of human ZBTB4. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.02% sodium azide |
Storage conditions |
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C |
RRID |
AB_11154166 |
Product Specific Information
A suggested positive control is SK-N-SH cell lysate.
PA5-21084 can be used with blocking peptide PEP-1198.
Target Information
The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: KAISO-L1; KAISO-like zinc finger protein 1; Zinc finger and BTB domain-containing protein 4
Gene Aliases: 2310026P19Rik; 9230111I22Rik; KAISO-L1; KIAA1538; mKIAA1538; ZBTB4; ZNF903
UniProt ID: (Human) Q9P1Z0, (Mouse) Q5F293
Entrez Gene ID: (Human) 57659, (Mouse) 75580
Molecular Function:
C2H2 zinc finger transcription factor
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
methyl-CpG binding
methyl-CpNpG binding
protein kinase binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support