Invitrogen

ZC3H8 Polyclonal Antibody

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ZC3H8 Antibody in Western Blot (WB) — Western blot analysis of ZC3H8 in HepG2 cells. Samples were incubated with ZC3H8 polyclonal antibody (Product # PA5-101962).

ZC3H8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ZC3H8 in mouse brain tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ZC3H8 polyclonal antibody (Product # PA5-101962) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ZC3H8 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ZC3H8 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZC3H8 polyclonal antibody (Product # PA5-101962) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ZC3H8, corresponding to a region within the internal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2851394

Target Information

Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: fetal liver zinc finger 1; fetal liver zinc finger 1; putative zinc finger protein FLIZ1; Fetal liver zinc finger protein 1; Zinc finger CCCH domain-containing protein 8; zinc finger CCCH type domain containing 8; zinc finger CCCH-type domain containing 8

Gene Aliases: AU020882; E130108N08Rik; Fliz1; ZC3H8; ZC3HDC8

UniProt ID: (Human) Q8N5P1, (Mouse) Q9JJ48

Entrez Gene ID: (Human) 84524, (Mouse) 57432

Function(s)