Invitrogen

ZC3H8 Polyclonal Antibody

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ZC3H8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZC3H8 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZC3H8 polyclonal antibody (Product # PA5-101962) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

ZC3H8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ZC3H8 in mouse brain tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ZC3H8 polyclonal antibody (Product # PA5-101962) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ZC3H8 Antibody in Western Blot (WB) — Western blot analysis of ZC3H8 in HepG2 cells. Samples were incubated with ZC3H8 polyclonal antibody (Product # PA5-101962).

Product Details

PA5-101962

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ZC3H8(Accession Q8N5P1), corresponding to amino acid residues I98-K126. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2851394

Target Information

Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: fetal liver zinc finger 1; fetal liver zinc finger 1; putative zinc finger protein FLIZ1; Fetal liver zinc finger protein 1; Zinc finger CCCH domain-containing protein 8; zinc finger CCCH type domain containing 8; zinc finger CCCH-type domain containing 8

Gene Aliases: AU020882; E130108N08Rik; Fliz1; ZC3H8; ZC3HDC8

UniProt ID: (Human) Q8N5P1, (Mouse) Q9JJ48

Entrez Gene ID: (Human) 84524, (Mouse) 57432

Molecular Function: zinc finger transcription factor