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Invitrogen
ZDHHC20 Polyclonal Antibody
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FIGURE: 1 / 3
ZDHHC20 Antibody (PA5-101778) in ICC/IF
Immunocytochemistry analysis of ZDHHC20 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZDHHC20 polyclonal antibody (Product # PA5-101778) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-101778
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZDHHC20(Accession Q5W0Z9), corresponding to amino acid residues M71-A121.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ZDHHC20.
Target Information
Catalyzes palmitoylation of Cys residues on target proteins (PubMed:27153536, PubMed:29326245). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acyltransferase ZDHHC20; DHHC domain-containing cysteine-rich protein 20; DHHC-20; DHHC-containing protein 20; membrane-associated DHHC24 zinc finger protein; probable palmitoyltransferase ZDHHC20; unnamed protein product; zinc finger DHHC domain-containing protein 20; zinc finger DHHC-type containing 20; zinc finger DHHC-type palmitoyltransferase 20; zinc finger, DHHC domain containing 20; zinc finger, DHHC-type containing 20
Gene Aliases: 4930542A17Rik; 4933421L13Rik; 5033406L14Rik; B230110O18Rik; DHHC-20; DHHC20; HK11; RGD1305755
UniProt ID: (Human) Q5W0Z9, (Mouse) Q5Y5T1
Entrez Gene ID: (Human) 253832, (Mouse) 75965, (Rat) 305923
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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