Invitrogen

ZDHHC9 Polyclonal Antibody

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ZDHHC9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZDHHC9 in COLO205 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZDHHC9 polyclonal antibody (Product # PA5-99617) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

ZDHHC9 Antibody in Western Blot (WB) — Western blot analysis of ZDHHC9 in COLO205 cells lysate. Samples were incubated with ZDHHC9 polyclonal antibody (Product # PA5-99617).

Product Details

PA5-99617

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human DHHC-9, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2818550

Target Information

The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: antigen MMSA-1; Asp-His-His-Cys domain containing protein 9; DHHC-9; DHHC9; membrane-associated DHHC9 zinc finger protein; Palmitoyltransferase ZDHHC9; Zinc finger DHHC domain-containing protein 9; Zinc finger protein 379; Zinc finger protein 380; zinc finger, DHHC domain containing 10; zinc finger, DHHC domain containing 9; zinc finger, DHHC-type containing 9

Gene Aliases: 6430508G22; 9530098M12Rik; CGI-89; CGI89; CXorf11; DHHC9; MMSA1; MRXSZ; UNQ261/PRO298; ZDHHC10; ZDHHC9; ZNF379; ZNF380

UniProt ID: (Human) Q9Y397, (Mouse) P59268

Entrez Gene ID: (Human) 51114, (Rat) 302808, (Mouse) 208884

Function(s)

peptidyl-L-cysteine S-palmitoylation protein palmitoylation

Process(es)

zinc ion binding palmitoyltransferase activity protein-cysteine S-palmitoyltransferase activity Ras palmitoyltransferase activity transferase activity transferase activity, transferring acyl groups metal ion binding