Immunofluorescent analysis of ZFAT showing staining in the nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a ZFAT polyclonal antibody (Product # PA5-32037) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 825 and 1083 of Human ZFAT|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32037 targets ZFAT in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-32037 immunogen is recombinant fragment corresponding to a region within amino acids 825 and 1083 of Human ZFAT.
This gene encodes a protein that likely binds DNA and functions as a transcriptional regulator involved in apoptosis and cell survival. This gene resides in a susceptibility locus for autoimmune thyroid disease (AITD) on chromosome 8q24. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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