Invitrogen

ZFYVE16 Polyclonal Antibody

Product Image Gallery

ZFYVE16 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZFYVE16 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ZFYVE16 polyclonal antibody (Product # PA5-100192) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

ZFYVE16 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ZFYVE16 in human thyroid cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ZFYVE16 polyclonal antibody (Product # PA5-100192) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ZFYVE16 Antibody in Western Blot (WB) — Western blot analysis of ZFYVE16 in mouse spleen tissue lysate (left lane: treated with blocking peptide). Samples were incubated with ZFYVE16 polyclonal antibody (Product # PA5-100192).

Product Details

PA5-100192

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ZFYVE16, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2815722

Target Information

The ZFYVE16 gene encodes endofin, an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Endofin; Endosomal-associated FYVE domain protein; Endosome-associated FYVE domain protein; endosome-associated FYVE-domain protein; protein phosphatase 1, regulatory subunit 69; Zinc finger FYVE domain-containing protein 16; zinc finger, FYVE domain containing 16

Gene Aliases: AI035632; B130024H06Rik; B130031L15; KIAA0305; mKIAA0305; PPP1R69; RGD1564784; ZFYVE16

UniProt ID: (Human) Q7Z3T8, (Mouse) Q80U44

Entrez Gene ID: (Human) 9765, (Mouse) 218441, (Rat) 499508

Function(s)

protein targeting to lysosome signal transduction vesicle organization endosomal transport regulation of endocytosis BMP signaling pathway

Process(es)

protein binding 1-phosphatidylinositol binding phosphatidylinositol-3,4,5-trisphosphate binding protein transporter activity metal ion binding