Invitrogen

ZIP14 Polyclonal Antibody

5 Published Figures

8 References

View all (11) ZIP14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (5)

ZIP14 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

ZIP14 Antibody (PA5-21077) in ICC/IF

Current issues in molecular biology 2022 - Immunofluorescence detection of ZIP14 transporter in normozoospermic ( a ) and asthenozoospermic ( c ) sperm samples using a confocal laser scanning microscope. Nc--negative control. Scale bar = 10 um. Average of total number of spermatozoa per ejaculate before processing was in normo- (625 x 10 6 /vol) and asthenozoospermia (367.5 x 10 6 /vol); For each type of sample, six randomly selected field areas wer... View More

ZIP14 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-21077

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to 16 amino acid synthetic peptide near the center of human ZIP14. The immunogen is located within amino acids 230-280 of ZIP14.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11157266

Product Specific Information

Suggested positive controls are human liver, mouse kidney, or rat kidney tissue lysates.

PA5-21077 can be used with blocking peptide PEP-1191.

Predicted species reactivity based on immunogen sequence: Bovine: (94%).

At least three isoforms of ZIP14 are known to exist; this antibody will detect all isoforms. ZIP14 antibody is predicted to not cross-react with other ZIP family members.

Target Information

The zinc transporter ZIP14, also known as SLC39A14, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP14 is a glycosylated multipass plasma membrane protein that belongs to the ZIP transporter subfamily LIV-1. ZIP14 has been shown to contribute to the hypozincemia of inflammation and infection and is regulated in the liver by IL-6. In addition to zinc, ZIP14 is also involved in the cellular uptake of non-transferrin-bound iron as well as iron bound to transferrin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cartilage tissue from sites of weight bearing in patients with osteoarthritis exhibits a differential phenotype with distinct chondrocytes subests.

RMD open

Di J,Chen Z,Wang Z,He T,Wu D,Weng C,Deng J,Mai L,Wang K,He L,Rong L

Published figure using ZIP14 polyclonal antibody (Product # PA5-21077) in Immunohistochemistry

Sun Oct 01 00:00:00 UTC 2023

Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.

Current issues in molecular biology

Protic I,Golic I,Vidakovic S,Korac B,Korac A

Published figure using ZIP14 polyclonal antibody (Product # PA5-21077) in Immunocytochemistry

Sun Jul 31 00:00:00 UTC 2022

Interleukin-6 triggers toxic neuronal iron sequestration in response to pathological α-synuclein.

Cell reports

Sterling JK,Kam TI,Guttha S,Park H,Baumann B,Mehrabani-Tabari AA,Schultz H,Anderson B,Alnemri A,Chou SC,Troncoso JC,Dawson VL,Dawson TM,Dunaief JL

Published figure using ZIP14 polyclonal antibody (Product # PA5-21077) in Western Blot

Tue Feb 15 00:00:00 UTC 2022

Transferrin receptor 1 ablation in satellite cells impedes skeletal muscle regeneration through activation of ferroptosis.

Journal of cachexia, sarcopenia and muscle

Ding H,Chen S,Pan X,Dai X,Pan G,Li Z,Mai X,Tian Y,Zhang S,Liu B,Cao G,Yao Z,Yao X,Gao L,Yang L,Chen X,Sun J,Chen H,Han M,Yin Y,Xu G,Li H,Wu W,Chen Z,Lin J,Xiang L,Hu J,Lu Y,Zhu X,Xie L

PA5-21077 was used in Western Blot to identify that Tfr1 expression is declined in muscles of aged mice.

Tue Jun 01 00:00:00 UTC 2021

Increased free Zn²⁺ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn²⁺ -transporters in heart failure.

Journal of cellular and molecular medicine

Olgar Y,Durak A,Tuncay E,Bitirim CV,Ozcinar E,Inan MB,Tokcaer-Keskin Z,Akcali KC,Akar AR,Turan B

Published figure using ZIP14 polyclonal antibody (Product # PA5-21077) in Western Blot

Thu Mar 01 00:00:00 UTC 2018

Prolonged stimulation of insulin release from MIN6 cells causes zinc depletion and loss of β-cell markers.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

Lawson R,Maret W,Hogstrand C

PA5-21077 was used in Immunocytochemistry to study the transcriptional effects of hyperglycaemia-induced zinc depletion in β-cell markers and β-cell dedifferentiation.

Sat Sep 01 00:00:00 UTC 2018

Conditional mouse models support the role of SLC39A14 (ZIP14) in Hyperostosis Cranialis Interna and in bone homeostasis.

PLoS genetics

Hendrickx G,Borra VM,Steenackers E,Yorgan TA,Hermans C,Boudin E,Waterval JJ,Jansen IDC,Aydemir TB,Kamerling N,Behets GJ,Plumeyer C,D'Haese PC,Busse B,Everts V,Lammens M,Mortier G,Cousins RJ,Schinke T,Stokroos RJ,Manni JJ,Van Hul W

PA5-21077 was used in Immunohistochemistry to reveal ZIP14 as a novel regulator of bone homeostasis, and that manipulating ZIP14 might be a therapeutic strategy for bone diseases.

Sun Apr 01 00:00:00 UTC 2018

Iron importers Zip8 and Zip14 are expressed in retina and regulated by retinal iron levels.

Experimental eye research

Sterling J,Guttha S,Song Y,Song D,Hadziahmetovic M,Dunaief JL

PA521077 was used in western blot to measure the expression of three ferrous iron importers, Dmt1, Zip8, and Zip14 in retinas

Wed Feb 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Factor for adipocyte differentiation 123; FAD-123; LIV-1 subfamily of ZIP zinc transporter 4; LZT-Hs4; Metal cation symporter ZIP14; solute carrier family 39 (metal ion transporter), member 14; solute carrier family 39 (zinc transporter), member 14; Solute carrier family 39 member 14; zinc transporter ZIP14; ZIP-14; Zrt- and Cig19; Zrt- and Irt-like protein 14; Zrt-, Irt-like protein 14

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Gene Aliases: cig19; FAD-123; Fad123; HMNDYT2; KIAA0062; LZT-Hs4; NET34; SLC39A14; ZIP-14; ZIP14

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UniProt ID: (Human) Q15043, (Mouse) Q75N73

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Entrez Gene ID: (Human) 23516, (Rat) 306009, (Mouse) 213053

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Function(s)

zinc ion transmembrane transporter activity ferrous iron transmembrane transporter activity metal ion transmembrane transporter activity secondary carrier transporter

Process(es)

cellular zinc ion homeostasis zinc II ion transmembrane transport zinc II ion transmembrane import ferrous iron transmembrane transport iron ion transport zinc II ion transport ferrous iron transport iron ion transmembrane transport transport ion transport metal ion transport transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZIP14 Polyclonal Antibody

ZIP14 Antibody (PA5-21077) in ICC/IF

Product Details

PA5-21077

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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