Invitrogen

ZIP14 Polyclonal Antibody

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ZIP14 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of ZIP14 in mouse liver tissue with ZIP14 Polyclonal Antibody (Product # PA5-21077) at 2.5 µg/mL.

ZIP14 Antibody in Western Blot (WB) — Western Blot analysis of ZIP14 in human spleen tissue lysate with ZIP14 Polyclonal Antibody (Product # PA5-21077) at (A) 1 and (B) 2 µg/mL.

Product Details

PA5-21077

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 3 publications 3 publications
Immunohistochemistry (IHC)	2.5 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A 16 amino acid peptide near the center of human ZIP14.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID	AB_11157266

Product Specific Information

A suggested positive control is human spleen tissue lysate.

PA5-21077 can be used with blocking peptide PEP-1191.

Target Information

The zinc transporter ZIP14, also known as SLC39A14, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP14 is a glycosylated multipass plasma membrane protein that belongs to the ZIP transporter subfamily LIV-1. ZIP14 has been shown to contribute to the hypozincemia of inflammation and infection and is regulated in the liver by IL-6. In addition to zinc, ZIP14 is also involved in the cellular uptake of non-transferrin-bound iron as well as iron bound to transferrin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transferrin receptor 1 ablation in satellite cells impedes skeletal muscle regeneration through activation of ferroptosis.

Journal of cachexia, sarcopenia and muscle

Ding H,Chen S,Pan X,Dai X,Pan G,Li Z,Mai X,Tian Y,Zhang S,Liu B,Cao G,Yao Z,Yao X,Gao L,Yang L,Chen X,Sun J,Chen H,Han M,Yin Y,Xu G,Li H,Wu W,Chen Z,Lin J,Xiang L,Hu J,Lu Y,Zhu X,Xie L

PA5-21077 was used in Western Blot to identify that Tfr1 expression is declined in muscles of aged mice.

Tue Jun 01 00:00:00 EDT 2021

Prolonged stimulation of insulin release from MIN6 cells causes zinc depletion and loss of β-cell markers.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

Lawson R,Maret W,Hogstrand C

PA5-21077 was used in Immunocytochemistry to study the transcriptional effects of hyperglycaemia-induced zinc depletion in β-cell markers and β-cell dedifferentiation.

Sat Sep 01 00:00:00 EDT 2018

Conditional mouse models support the role of SLC39A14 (ZIP14) in Hyperostosis Cranialis Interna and in bone homeostasis.

PLoS genetics

Hendrickx G,Borra VM,Steenackers E,Yorgan TA,Hermans C,Boudin E,Waterval JJ,Jansen IDC,Aydemir TB,Kamerling N,Behets GJ,Plumeyer C,D'Haese PC,Busse B,Everts V,Lammens M,Mortier G,Cousins RJ,Schinke T,Stokroos RJ,Manni JJ,Van Hul W

PA5-21077 was used in Immunohistochemistry to reveal ZIP14 as a novel regulator of bone homeostasis, and that manipulating ZIP14 might be a therapeutic strategy for bone diseases.

Sun Apr 01 00:00:00 EDT 2018

Increased free Zn²⁺ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn²⁺ -transporters in heart failure.

Journal of cellular and molecular medicine

Olgar Y,Durak A,Tuncay E,Bitirim CV,Ozcinar E,Inan MB,Tokcaer-Keskin Z,Akcali KC,Akar AR,Turan B

Published figure using ZIP14 polyclonal antibody (Product # PA5-21077) in Western Blot

Thu Mar 01 00:00:00 EST 2018

Iron importers Zip8 and Zip14 are expressed in retina and regulated by retinal iron levels.

Experimental eye research

Sterling J,Guttha S,Song Y,Song D,Hadziahmetovic M,Dunaief JL

PA521077 was used in western blot to measure the expression of three ferrous iron importers, Dmt1, Zip8, and Zip14 in retinas

Wed Feb 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Factor for adipocyte differentiation 123; FAD-123; LIV-1 subfamily of ZIP zinc transporter 4; LZT-Hs4; Metal cation symporter ZIP14; solute carrier family 39 (metal ion transporter), member 14; solute carrier family 39 (zinc transporter), member 14; Solute carrier family 39 member 14; zinc transporter ZIP14; ZIP-14; Zrt- and Cig19; Zrt- and Irt-like protein 14; Zrt-, Irt-like protein 14

Gene Aliases: cig19; FAD-123; Fad123; HMNDYT2; KIAA0062; LZT-Hs4; NET34; SLC39A14; ZIP-14; ZIP14

UniProt ID: (Human) Q15043, (Mouse) Q75N73

Entrez Gene ID: (Human) 23516, (Rat) 306009, (Mouse) 213053

Molecular Function: secondary carrier transporter