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Invitrogen
ZIPK Polyclonal Antibody
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FIGURE: 1 / 2
ZIPK Antibody (PA5-19902) in WB
Western blot was performed using Anti-ZIPK Polyclonal Antibody (Product # PA5-19902) and a 52 kDa band corresponding to DAPK3 was observed across cell lines tested except LNCaP which is reported to be low. A doublet banding pattern is observed, which probably is due to autophosphorylation of the DAPK3 which is reported. Modified whole cell extracts (1% SDS) (30 µg lysate) of DU 145 (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), LNCaP (Lane 4) and U-87 MG (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX).... View More
Product Details
PA5-19902
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to amino acids near the center of human ZIP kinase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot applications, this antibody detects a band at ~40kDa, which represents the pro-enzyme of DNase II. A suggested positive control is human spleen tissue lysate.
PA5-19902 can be used with blocking peptide PEP-0028.
Target Information
DAPK3 is a serine/threonine kinase which is functionally linked to cell motility, smooth muscle contractility and cell death processes. DAPK3 is encoded by ZIPK and is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAP kinase 3; DAP-like kinase; DAP3K; death-associated kinase 3; Death-associated like kinase; Death-associated protein kinase 3; Dlk; MYPT1 kinase; ZIP kinase; ZIP kinase isoform; ZIP-kinase; Zipper-interacting protein kinase
Gene Aliases: DAPK3; Dapkl; DLK; ZIP; ZIPK
UniProt ID: (Human) O43293, (Rat) O88764, (Mouse) O54784
Entrez Gene ID: (Human) 1613, (Rat) 64391, (Mouse) 13144
protein serine/threonine kinase activity
protein binding
ATP binding
protein C-terminus binding
cAMP response element binding protein binding
Rho GTPase binding
identical protein binding
protein homodimerization activity
leucine zipper domain binding
calmodulin-dependent protein kinase activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
cytokinesis
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
apoptotic process
regulation of smooth muscle contraction
regulation of mitotic nuclear division
regulation of mitotic cell cycle
regulation of cell shape
regulation of autophagy
covalent chromatin modification
negative regulation of translation
neuron differentiation
positive regulation of cell migration
intracellular signal transduction
regulation of apoptotic process
positive regulation of apoptotic process
regulation of myosin II filament organization
protein autophosphorylation
regulation of focal adhesion assembly
cellular response to interferon-gamma
positive regulation of canonical Wnt signaling pathway
apoptotic signaling pathway
regulation of cell motility
regulation of actin cytoskeleton reorganization
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
chromatin modification
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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