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Invitrogen
ZNF143 Polyclonal Antibody
FIGURE: 1 / 4
ZNF143 Antibody (PA5-116147) in ICC/IF
Immunocytochemistry analysis of ZNF143 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116147) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116147
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZNF143(Accession P52747), corresponding to amino acid residues I73-S123.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900781
Product Specific Information
Antibody detects endogenous levels of total ZNF143.
Target Information
Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hStaf; mStaf; selenocysteine tRNA gene transcription activating factor; Selenocysteine tRNA gene transcription-activating factor; SPH-binding factor; transcriptional activator Staf; Zfp-143; Zinc finger protein 143; zinc finger protein 80, related sequence 1
Gene Aliases: AA959806; AU015869; D7Ertd805e; KRAB14; pHZ-1; SBF; STAF; Zfp143; Zfp79; Zfp80-rs1; ZNF143
UniProt ID: (Human) P52747, (Mouse) O70230, (Rat) Q5XIU2
Entrez Gene ID: (Human) 7702, (Mouse) 20841, (Rat) 361627
regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase III promoter
transcription from RNA polymerase II promoter
transcription from RNA polymerase III promoter
snRNA transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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