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Invitrogen
ZNF148 Polyclonal Antibody
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FIGURE: 1 / 3
ZNF148 Antibody (PA5-99320) in ICC/IF
Immunofluorescent analysis of ZNF148 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZNF148 polyclonal antibody (Product # PA5-99320) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-99320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZNF148(Accession Q9UQR1), corresponding to amino acid residues L64-V114.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818253
Product Specific Information
Antibody detects endogenous levels of total ZNF148.
Target Information
ZNF148 is involved in transcriptional regulation. ZNF148 represses the transcription of a number of genes including gastrin, stromelysin and enolase. ZNF148 binds to the G-rich box in the enhancer region of these genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta enolase repressor factor 1; CACCC box-binding protein; CLL-associated antigen KW-10; G-rich box-binding protein; Transcription factor BFCOL1; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; Zinc finger protein 148
Gene Aliases: 2210405J08Rik; AI480666; AW045217; BERF-1; BFCOL1; HT-BETA; pHZ-52; ZBP-89; ZBP89; ZFP148; ZNF148
UniProt ID: (Human) Q9UQR1, (Rat) Q62806, (Mouse) Q61624
Entrez Gene ID: (Human) 7707, (Rat) 58820, (Mouse) 22661
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
sequence-specific DNA binding
metal ion binding
transcription regulatory region sequence-specific DNA binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein complex assembly
cellular defense response
gamete generation
negative regulation of gene expression
substantia nigra development
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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