Invitrogen

ZNF148 Polyclonal Antibody

Product Image Gallery

ZNF148 Antibody in Western Blot (WB) — Western blot analysis of ZNF148 in various samples (Lane 1: rat brain treated with blocking peptide, Lane 2: rat brain, Lane 3: mouse brain). Samples were incubated with ZNF148 polyclonal antibody (Product # PA5-99320).

ZNF148 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded ZNF148 in human Kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with ZNF148 polyclonal antibody (Product # PA5-99320) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ZNF148 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ZNF148 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZNF148 polyclonal antibody (Product # PA5-99320) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ZNF148, corresponding to a region within N-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2818253

Target Information

ZNF148 is involved in transcriptional regulation. ZNF148 represses the transcription of a number of genes including gastrin, stromelysin and enolase. ZNF148 binds to the G-rich box in the enhancer region of these genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta enolase repressor factor 1; CACCC box-binding protein; CLL-associated antigen KW-10; G-rich box-binding protein; Transcription factor BFCOL1; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; Zinc finger protein 148

Gene Aliases: 2210405J08Rik; AI480666; AW045217; BERF-1; BFCOL1; HT-BETA; pHZ-52; ZBP-89; ZBP89; ZFP148; ZNF148

UniProt ID: (Human) Q9UQR1, (Rat) Q62806, (Mouse) Q61624

Entrez Gene ID: (Human) 7707, (Rat) 58820, (Mouse) 22661

Function(s)