Invitrogen

ZNF207 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (9) ZNF207 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ZNF207 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of ZNF207 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a ZNF207 Polyclonal Antibody (Product # PA5-53535).

ZNF207 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ZNF207 in human colon, kidney, lymph node and testis using ZNF207 Polyclonal Antibody (Product # PA5-53535) (A) shows similar protein distribution across tissues to an independent ZNF207 Polyclonal Antibody (B).

ZNF207 Antibody in Western Blot (WB) — Western blot analysis of ZNF207 in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using a ZNF207 Polyclonal Antibody (Product # PA5-53535). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

ZNF207 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. Hek293 cells were transfected with ZNF207 siRNA and decrease in signal intensity was observed in western blot application using ZNF207 Polyclonal Antibody (Product # PA5-53535).

Product Details

PA5-53535

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ZNF207

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2649996

Product Specific Information

Immunogen sequence: KPATLTTTSA TSKLIHPDED ISLEERRAQL PKYQRNLPRP GQAPIGNPPV GPIGGMMPPQ PGIPQQQGMR PPMPPHGQYG GHHQGMPGYL PGAMPPYGQG P

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 99%.

Target Information

ZNF207 is a new candidate transcription factor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 49 kDa zinc finger protein; BUB3-interacting and GLEBS motif-containing protein ZNF207; BuGZ; Zinc finger protein 207

Gene Aliases: 8430401D15Rik; BUGZ; hBuGZ; Zep; Zfp207; ZNF207

UniProt ID: (Human) O43670, (Mouse) Q9JMD0

Entrez Gene ID: (Human) 7756, (Mouse) 22680, (Rat) 303763

Molecular Function: DNA metabolism protein

Function(s)

mitotic sister chromatid segregation microtubule bundle formation regulation of transcription, DNA-templated mitotic spindle assembly checkpoint attachment of spindle microtubules to kinetochore microtubule polymerization protein stabilization cell division regulation of chromosome segregation mitotic spindle assembly cell cycle chromosome segregation mitotic nuclear division

Process(es)

DNA binding transcription factor activity, sequence-specific DNA binding protein binding microtubule binding heparin binding zinc ion binding poly(A) RNA binding metal ion binding