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ZNF281 Antibody (PA5-116145) in IHC (P)
Immunohistochemistry analysis of ZNF281 in human colorectal cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116145) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-116145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZNF281(Accession Q9Y2X9), corresponding to amino acid residues Q396-S446.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900779
Product Specific Information
Antibody detects endogenous levels of total Zfp281.
Target Information
ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GC-box-binding zinc finger protein 1; Transcription factor ZBP-99; Zinc finger DNA-binding protein 99; Zinc finger protein 281; ZNP-99 transcription factor
Gene Aliases: GZP1; ZBP-99; ZBP99; ZNF281; ZNP-99
UniProt ID: (Human) Q9Y2X9
Entrez Gene ID: (Human) 23528, (Rat) 305083
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
embryonic body morphogenesis
negative regulation of gene expression
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
stem cell differentiation
regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
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