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ZNF313 Antibody (PA5-54344) in ICC/IF
Immunofluorescence analysis of ZNF313 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ZNF313 Polyclonal Antibody (Product # PA5-54344, 0.8 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:... View More
Product Details
PA5-54344
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human ZNF313. Recombinant protein control fragment (Product #RP-91628).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2650060
Product Specific Information
Immunogen sequence: SALAPGVRAV ELERQIESTE TSCHGCRKNF FLSKIRSHVA TCSKYQNYIM EGVKATIKDA SLQPRNVPNR YTFPCPYCPE KNFDQEGLVE HCKLFHSTDT KSVVCPICAS MPW
Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 91%.
Target Information
ZNF313 is a novel human zinc finger protein gene that contains both ring finger and C(2)H(2) domain. It may play a role in spermatogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF114; RING finger protein 114; RING-type E3 ubiquitin transferase RNF114; Zinc finger protein 228; Zinc finger protein 313
Gene Aliases: 1110008J21Rik; AI225886; AW549494; PSORS12; RNF114; Zfp228; Zfp313; ZNF228; ZNF313
UniProt ID: (Human) Q9Y508, (Mouse) Q9ET26, (Rat) Q6J2U6
Entrez Gene ID: (Human) 55905, (Mouse) 81018, (Rat) 362277
ubiquitin-protein transferase activity
zinc ion binding
ligase activity
ubiquitin conjugating enzyme binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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