Invitrogen

ZNF75D Polyclonal Antibody

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ZNF75D Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZNF75D in HUVEC cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZNF75D polyclonal antibody (Product # PA5-101121) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

ZNF75D Antibody in Western Blot (WB) — Western blot analysis of ZNF75D in HUVEC cells. Samples were incubated with ZNF75D polyclonal antibody (Product # PA5-101121).

Product Details

PA5-101121

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ZNF75D(Accession P51815), corresponding to amino acid residues C300-W329. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2850565

Target Information

Zinc finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger protein 75 (ZNF75), also known as ZNF82, is a 289 amino acid member of the Krueppel C2H2-type zinc finger protein family. Localized to the nucleus, ZNF75 contains five C2H2- type zinc fingers and one KRAB domain through which it is thought to be involved in DNA-binding and transcriptional regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.