Immunofluorescent analysis of ZNRF1 in paraffin-embedded mouse cerebral cortex tissue following microwave antigen retrieval with a citrate buffer, pH 6, using a ZNRF1 polyclonal antibody (Product# PA5-37982) at a dilution of 10ug/ml. Detection of ZNRF1 was performed using a biotinylated anti-goat secondary antibody followed by streptavidin-Alexa Fluor 488 (green, left). Neurofilament M was stained using a labeled secondary antibody at a dilution of 1:100 (red, center), and a merged image is shown on the right.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-SDSTYAHGNGYQET, from the internal region of mouse Znrf1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human, rat, dog, pig and cow based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.