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Invitrogen
ZNRF3 Polyclonal Antibody
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FIGURE: 1 / 3
ZNRF3 Antibody (PA5-143891) in ICC/IF
Immunocytochemistry analysis of ZNRF3 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ZNRF3 Polyclonal antibody (Product # PA5-143891) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-143891
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ZNRF3 recombinant protein (Position: R54-E913).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human CACO-2 whole cell, human A549 whole cell, human Hacat whole cell. ICC/IF: U20S cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The ZNRF3 gene encodes a transmembrane E3 ubiquitin ligase that plays a critical role in regulating the Wnt signaling pathway, which is essential for various biological processes, including embryonic development and cell proliferation. ZNRF3 functions by negatively regulating this pathway through ubiquitination and degradation of Frizzled receptors, thereby suppressing the Wnt signaling. Located on chromosome 22, ZNRF3 acts as a tumor suppressor gene by inhibiting oncogenic Wnt signaling and has been implicated in the formation and progression of several cancers, particularly colorectal cancer. Moreover, ZNRF3 interacts with R-spondin proteins which are known to augment Wnt signaling by targeting ZNRF3 for degradation, thus facilitating cell differentiation and proliferation. Alterations in ZNRF3 expression or mutations can lead to aberrant Wnt signaling, contributing to tumorigenesis. Additionally, ZNRF3's role extends to its participation in lipid metabolism and maintenance of bone homeostasis. It has been studied as a potential therapeutic target due to its central involvement in these regulatory pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bK175E3.6; CTA-292E10.6; E3 ubiquitin-protein ligase ZNRF3; novel C3HC4 type Zinc finger (ring finger); RING finger protein 203; RING-type E3 ubiquitin transferase ZNRF3; unnamed protein product; Zinc/RING finger protein 3
Gene Aliases: BK747E2.3; KIAA1133; RNF203; ZNRF3
UniProt ID: (Human) Q9ULT6
Entrez Gene ID: (Human) 84133
ubiquitin-dependent protein catabolic process
Wnt signaling pathway
protein ubiquitination
negative regulation of Wnt signaling pathway
Wnt receptor catabolic process
limb development
regulation of canonical Wnt signaling pathway
stem cell proliferation
negative regulation of canonical Wnt signaling pathway
regulation of non-canonical Wnt signaling pathway
negative regulation of non-canonical Wnt signaling pathway
regulation of Wnt signaling pathway, planar cell polarity pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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