Proteintech

ZO-1 Polyclonal Antibody

Product Image Gallery

ZO-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HUVEC cells using 21773-1-AP (ZO-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ZO-1 Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 21773-1-AP (ZO-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ZO-1 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug ZO-1 antibody (21773-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

ZO-1 Antibody in Immunoprecipitation (IP) — IP result of anti-ZO-1 (IP:21773-1-AP, 5ug; Detection:21773-1-AP 1:800) with HEK-293 cells lysate 1800ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 79 publications 79 publications

Immunohistochemistry (IHC)

Assay-dependent

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 31 publications 31 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZO-1 Fusion Protein Ag16454

Conjugate

Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

Gene Aliases: I79_005485; TJP1; ZO-1; ZO1

UniProt ID: (Dog) O97758, (Human) Q07157, (Mouse) P39447

Entrez Gene ID: (Dog) 403752, (Human) 7082, (Mouse) 21872, (Rat) 292994, (Chinese hamster) 100773110

Molecular Function: tight junction