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Invitrogen
ZO-2 Polyclonal Antibody
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FIGURE: 1 / 87
ZO-2 Antibody (71-1400) in ICC/IF
Immunofluorescent analysis of ZO2/TJP2 Antibody was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO2/TJP2 Antibody (Product # 71-1400) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at roo... View More
Product Details
71-1400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Published species
Dog,
Fish,
Human,
Mouse,
Pig,
Plant,
Rabbit,
Rat,
Sheep,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the central portion of the ZO-2 protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Friedreich ataxia region gene X104 (tight junction protein ZO-2); Tight junction protein 2; Tight junction protein ZO-2; Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2); unnamed protein product; X104; ZO-2A; ZO-2C; zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2
Gene Aliases: C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1; PFIC4; TJP2; X104; ZO-2; ZO2
UniProt ID: (Dog) Q95168, (Human) Q9UDY2, (Mouse) Q9Z0U1
Entrez Gene ID: (Dog) 403854, (Human) 9414, (Mouse) 21873
homotypic cell-cell adhesion
maintenance of blood-brain barrier
cell-cell junction organization
GMP metabolic process
GDP metabolic process
intestinal absorption
establishment of endothelial intestinal barrier
regulation of membrane permeability
cell-cell adhesion
protein localization to cell-cell junction
positive regulation of maintenance of permeability of blood-brain barrier
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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