Invitrogen

ZONAB Polyclonal Antibody

Product Image Gallery

ZONAB Antibody in Immunofluorescence (IF) — Indirect immunofluorescent staining of mouse liver using our ZONAB (Mid) Polyclonal Antibody, Rabbit (Product # 40-2800). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada

ZONAB Antibody in Western Blot (WB) — Rabbit anti-ZONAB (Mid) polyclonal antibody was used at 2 µg/mL. ZONAB could be detected after loading 20 µg of ms testes tissue lysate from a wild-type mouse on gel and detection by western blot (lane 1). As a negative control, 20 µg of testes tissue lysate from a knock-out mouse was loaded in lane 2.

ZONAB Antibody — Published figure using ZONAB polyclonal antibody (Product # 40-2800)

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

2-3 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

2-3 µg/mL

Immunoprecipitation (IP)

1-2 µg

Western Blot (WB)

3-4 µg/mL

Published Applications

Miscellaneous PubMed (MISC)

See 1 publication below

Immunocytochemistry (ICC)

See 1 publication below

Western Blot (WB)

See 5 publications below

Immunofluorescence (IF)

See 4 publications below

Immunohistochemistry (IHC)

See 2 publications below

Immunomicroscopy (IM)

See 1 publication below

Immunoprecipitation (IP)

See 1 publication below

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Dog, Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of mouse ZONAB (ZO-1 associated nucleic acid-binding protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

RRID

AB_2533460

Product Specific Information

This antibody reacts with an internal region of the ZONAB protein. On Western blots, it identifies a major band at ~65 kDa and a minor band at ~54 kDa. The antibody is directed towards a region of ZONAB common to both the long and the alternatively-spliced short form. During development, reactivity was confirmed using mouse testis tissue homogenates by Western blotting and labeling of mouse testis tissue using immunofluorescence. All signals were absent in knockout mice, further confirming the reactivity with ZONAB.

Target Information

ZONAB (ZO-1 associated nucleic acid-binding) is a Y-box transcription factor whose nuclear localization and transcriptional activity is regulated by the tight junction-associated candidate tumor suppressor ZO-1. Two ZONAB isoforms are known, a long isoform (ZONAB B) and an alternatively spliced shorter isoform (ZONAB A) that lacks 68 amino acids. ZONAB regulates the expression of the tyrosine kinase receptor HER2, and appears to act in combination with ZO-1 to regulate HER2 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cold shock domain protein A; cold shock domain-containing protein A; DNA-binding protein A; muscle Y-box protein YB2; RYB-A; Y-box protein 3; Y-box-binding protein 3; Y-box-binding protein A; ZO-1 associated nucleic acid binding protein; ZONAB A; ZONAB B; zonula occludens 1 associated nucleic acid binding protein

Gene Aliases: Csda; Dbpa; Dpba; MSY3; MSY4; oxyR; Yb2

Entrez Gene ID: (Rat) 83807, (Mouse) 56449

Function(s)