Immunofluorescent analysis of ZXDA showing staining in the nucleus of SK-N-SH cells. SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a ZXDA polyclonal antibody (Product # PA5-31856) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 114 and 305 of Human ZXDA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31856 targets ZXDA in WB applications and shows reactivity with Human samples.
The PA5-31856 immunogen is recombinant protein fragment corresponding to a region within amino acids 114 and 305 of Human ZXDA.
This gene encodes one of two duplicated zinc finger genes on chromosome Xp11. This gene is the telomeric copy; GeneID 158586 ZXDB is the more centromeric copy. The two genes have 98% nucleotide sequence similarity, and the predicted proteins contain 10 tandem zinc finger motifs.
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