Invitrogen

alpha-1a Adrenergic Receptor Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

alpha-1a Adrenergic Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of alpha-1 Adrenergic Receptor (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Alpha-1 Adrenergic Receptor polyclonal antibody (Product # PA1-047) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

alpha-1a Adrenergic Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of alpha-1 Adrenergic Receptor showing positive staining in the cytoplasm and membrane of paraffin-treated Human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a alpha-1 Adrenergic Receptor polyclonal antibody (Product # PA1-047) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

alpha-1a Adrenergic Receptor Antibody in Immunohistochemistry (IHC) — Immunolocalization of A1AR in mouse distal convoluted tubule using Product # PA1-047.

alpha-1a Adrenergic Receptor Antibody in Immunohistochemistry - Free Floating (IHC (Free)) — Published figure using alpha-1a Adrenergic Receptor polyclonal antibody (Product # PA1-047) in Immunohistochemistry - Free Floating

Product Details

PA1-047

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Rat, Zebrafish

Published species

Cat, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2273801

Product Specific Information

PA1-047 detects alpha-1 adrenergic receptor (A1AR) from human, mouse, rat, zebra fish (Danio rerio) and Drosophila samples.

PA1-047 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing the A1AR from mouse kidney membrane preparations, mouse liver, HeLa cell lysates, zebra fish and drosophila samples. Immunofluorescence staining of A1AR in mouse kidney distal tubule cells with PA1-047 results in staining of the plasma membrane.

The PA1-047 immunogen is a synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR. This sequence is 100% conserved in all A1AR subtypes examined. PA1-047 immunizing peptide (Cat. # PEP-216) is available for use in neutralization and control experiments.

Target Information

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibiting α1-adrenergic receptor signaling pathway ameliorates AD-type pathologies and behavioral deficits in APPswe/PS1 mouse model.

Journal of neurochemistry

Yu ZY,Yi X,Wang YR,Zeng GH,Tan CR,Cheng Y,Sun PY,Liu ZH,Wang YJ,Liu YH

PA1-047 was used in Western Blotting to indicate that inhibition of α1-ARs signaling pathway lowers brain amyloid burden, attenuates downstream pathologies and rescues behavioral deficits in APPswe/PS1 mice.

Sun May 01 00:00:00 EDT 2022

Singular versus combinatory glucose-sensitive signal control of metabolic sensor protein profiles in hypothalamic astrocyte cultures from each sex.

Translational neuroscience

Alhamyani A,Napit PR,Bheemanapally K,Sylvester PW,Briski KP

PA1-047 was used in Immunohistochemistry-immunofluorescence to show that DEX and NE regulation of GLUT2 is sex-monomorphic, but both stimuli impose divergent sex-specific effects on AMPK and pAMPK.

Sat Jan 01 00:00:00 EST 2022

Quantification of brainstem norepinephrine relative to vocal impairment and anxiety in the Pink1-/- rat model of Parkinson disease.

Behavioural brain research

Hoffmeister JD,Kelm-Nelson CA,Ciucci MR

PA1-047 was used in Immunohistochemistry-immunofluorescence to demonstrate statistically significant relationships among vocal impairment, anxiety, and brainstem norepinephrine in the Pink1-/- rat model of PD.

Fri Sep 24 00:00:00 EDT 2021

Norepinephrine Regulation of Adrenergic Receptor Expression, 5' AMP-Activated Protein Kinase Activity, and Glycogen Metabolism and Mass in Male Versus Female Hypothalamic Primary Astrocyte Cultures.

ASN neuro

Ibrahim MMH,Bheemanapally K,Sylvester PW,Briski KP

PA1-047 was used in Western Blot to investigate the premise that NE imposes sex-specific direct control of AMPK activity and glycogen mass and metabolism in these glia, using a rat hypothalamic astrocyte primary culture model.

Wed Sep 15 00:00:00 EDT 2021

Carotid smooth muscle contractility changes after severe burn.

Scientific reports

DeSpain K,Rosenfeld CR,Huebinger R,Wang X,Jay JW,Radhakrishnan RS,Wolf SE,Song J

PA1-047 was used in Immunohistochemistry to investigate the changes in carotid artery contractility and relaxation following a severe burn.

Fri Sep 10 00:00:00 EDT 2021

Noradrenergic α1-Adrenoceptor Actions in the Primate Dorsolateral Prefrontal Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Datta D,Yang ST,Galvin VC,Solder J,Luo F,Morozov YM,Arellano J,Duque A,Rakic P,Arnsten AFT,Wang M

PA1-047 was used in Immunohistochemistry to study the effects of a1-AR manipulation on neuronal firing in post-traumatic stress disorder.

Wed Apr 03 00:00:00 EDT 2019

The β₂ agonist terbutaline specifically decreases pulmonary arterial pressure under normoxia and hypoxia via α adrenoceptor antagonism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Neumann V,Knies R,Seidinger A,Simon A,Lorenz K,Matthey M,Breuer J,Wenzel D

PA1-047 was used in Western Blotting to provide a novel option to reduce pulmonary arterial pressure in pulmonary hypertension.

Tue May 01 00:00:00 EDT 2018

Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons.

Brain structure & function

Hormigo S,Gómez-Nieto R,Sancho C,Herrero-Turrión J,Carro J,López DE,Horta-Júnior JACE

PA1-047 was used in Immunohistochemistry to support the participation of LC in ASR and PPI, and contributes toward a better understanding of sex-related differences observed in somatosensory gating paradigms.

Wed Nov 01 00:00:00 EDT 2017

Predator Stress-Induced CRF Release Causes Enduring Sensitization of Basolateral Amygdala Norepinephrine Systems that Promote PTSD-Like Startle Abnormalities.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Rajbhandari AK,Baldo BA,Bakshi VP

Published figure using alpha-1a Adrenergic Receptor polyclonal antibody (Product # PA1-047) in Immunofluorescence

Wed Oct 21 00:00:00 EDT 2015

Loss of motoneurons in the ventral compartment of the rat hypoglossal nucleus following early postnatal exposure to alcohol.

Journal of chemical neuroanatomy

Stettner GM,Kubin L,Volgin DV

PA1-047 was used in immunohistochemistry to study motoneuron loss in the rat hypoglossal nucleus venral compartment in response to alcohol exposure in the early post-natal period

Sun Sep 01 00:00:00 EDT 2013

Enhanced role of adenosine A(2A) receptors in the modulation of LTP in the rat hippocampus upon ageing.

The European journal of neuroscience

Costenla AR,Diógenes MJ,Canas PM,Rodrigues RJ,Nogueira C,Maroco J,Agostinho PM,Ribeiro JA,Cunha RA,de Mendonça A

PA1-047 was used in immunocytochemistry to investigate the role of adenosine A2A receptors in hippocampal synaptic plasticity in rats

Fri Jul 01 00:00:00 EDT 2011

Left ventricular dysfunction in murine models of heart failure and in failing human heart is associated with a selective decrease in the expression of caveolin-3.

Journal of cardiac failure

Feiner EC,Chung P,Jasmin JF,Zhang J,Whitaker-Menezes D,Myers V,Song J,Feldman EW,Funakoshi H,Degeorge BR,Yelamarty RV,Koch WJ,Lisanti MP,McTiernan CF,Cheung JY,Bristow MR,Chan TO,Feldman AM

PA1-047 was used in western blot to investigate the relationship between caveolin-3 expression and left ventricular dysfunction

Tue Mar 01 00:00:00 EST 2011

Blockade of adenosine A(1) receptors prevents methylphenidate-induced impairment of object recognition task in adult mice.

Progress in neuro-psychopharmacology & biological psychiatry

Mioranzza S,Costa MS,Botton PH,Ardais AP,Matte VL,Espinosa J,Souza DO,Porciúncula LO

PA1-047 was used in western blot to investigate the involvement of adenosine A1 receptor system in the cognitive enhancing effect of methylphenidate

Sat Jan 15 00:00:00 EST 2011

Adenosine receptor A1 regulates polymorphonuclear cell trafficking and microvascular permeability in lipopolysaccharide-induced lung injury.

Journal of immunology (Baltimore, Md. : 1950)

Ngamsri KC,Wagner R,Vollmer I,Stark S,Reutershan J

PA1-047 was used in western blot to investigate the role of adenosine receptor A1 in lipopolysaccharide-induced lung injury

Fri Oct 01 00:00:00 EDT 2010

Caffeine and an adenosine A(2A) receptor antagonist prevent memory impairment and synaptotoxicity in adult rats triggered by a convulsive episode in early life.

Journal of neurochemistry

Cognato GP,Agostinho PM,Hockemeyer J,Müller CE,Souza DO,Cunha RA

PA1-047 was used in immunohistochemistry to investigate the effect of kainate on the status of hippocampal adenosine receptors

Fri Jan 01 00:00:00 EST 2010

Alpha 1-noradrenergic system role in increased motivation for cocaine intake in rats with prolonged access.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology

Wee S,Mandyam CD,Lekic DM,Koob GF

PA1-047 was used in immunohistochemistry to investigate the possible effect of neuroadaptation in the central noradrenergic system on increased cocaine self-administration in rats with extended access

Tue Apr 01 00:00:00 EDT 2008

Adenosine deamination sustains dendritic cell activation in inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Desrosiers MD,Cembrola KM,Fakir MJ,Stephens LA,Jama FM,Shameli A,Mehal WZ,Santamaria P,Shi Y

PA1-047 was used in flow cytometry to investigate the function of adenosine deaminase in dendritic inflammatory responses

Wed Aug 01 00:00:00 EDT 2007

Activation of subfornical organ neurons in rats through pre- and postsynaptic alpha-adrenoceptors.

American journal of physiology. Regulatory, integrative and comparative physiology

Honda E,Ono K,Kataoka S,Inenaga K

PA1-047 was used in immunohistochemistry to suggest that activation of subfornical organ neurons in rats are activated postsynaptically

Thu Jun 01 00:00:00 EDT 2006

Biochemistry and pharmacology of epitope-tagged alpha(1)-adrenergic receptor subtypes.

The Journal of pharmacology and experimental therapeutics

Vicentic A,Robeva A,Rogge G,Uberti M,Minneman KP

PA1-047 was used in western blot to study the biochemistry and pharmacology of epitope-tagged alpha (1)-adrenergic receptor subtypes in HEK293 cells

Mon Jul 01 00:00:00 EDT 2002

N-(2-Chloroethyl)-N-ethyl-2-bromobenzylamine reduces intracellular calcium response to noradrenaline in rat visual cortex.

Neuroscience

Yamamoto M,Imamura K,Kobayashi M,Nakadate K,Yokoyama C,Watanabe Y,Yamamoto M,Negi A

PA1-047 was used in immunohistochemistry to study the effect of the noradrenergic neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine on intracellular calcium responses to noradrenaline in the visual cortex

Mon Dec 17 00:00:00 EST 2001

Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.

Neuroscience research

Nakadate K,Imamura K,Watanabe Y

PA1-047 was used in immunohistochemistry to study the regulation of adrenergic receptors by sensory afferents in kitten brains

Fri Jun 01 00:00:00 EDT 2001

Transcriptional regulation of alpha1-adrenoceptor gene in the rat liver during different phases of sepsis.

Biochimica et biophysica acta

Dong LW,Yang J,Tong LJ,Tang C,Liu MS

PA1-047 was used in western blot to study the alpha1 adrenoceptor protein expression in the rat liver during different phases of sepsis

Wed Feb 24 00:00:00 EST 1999

Bioinformatics

Protein Aliases: ADA1; adrenergic alpha 1c receptor; adrenergic receptor alpha 1c subtype; adrenergic receptor, alpha 1a; adrenergic receptor, alpha 1c; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1Aa, receptor; adrenoceptor alpha 1A; alpha 1A-adrenoceptor; alpha 1A-adrenoreceptor; alpha 1C-adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoceptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; Alpha1C-AR; G protein coupled receptor

Gene Aliases: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR; fc18h12; wu:fc18h12

UniProt ID: (Human) P35348, (Mouse) P97718, (Rat) P43140

Entrez Gene ID: (Human) 148, (Mouse) 11549, (Rat) 29412, (Zebrafish) 798498

Molecular Function: G-protein coupled receptor