Invitrogen

alpha-1a Adrenergic Receptor Polyclonal Antibody

Product Image Gallery

alpha-1a Adrenergic Receptor Antibody — Published figure using alpha-1a Adrenergic Receptor polyclonal antibody (Product # PA1-047)

alpha-1a Adrenergic Receptor Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of alpha-1 Adrenergic Receptor (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Alpha-1 Adrenergic Receptor polyclonal antibody (Product # PA1-047) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

alpha-1a Adrenergic Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of alpha-1 Adrenergic Receptor showing positive staining in the cytoplasm and membrane of paraffin-treated Human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a alpha-1 Adrenergic Receptor polyclonal antibody (Product # PA1-047) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:10-1:1000

Immunofluorescence (IF)

1:10-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:400

Published Applications

Immunohistochemistry - Free Floating (IHC (Free))

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (IHC)

See 7 publications below

Immunocytochemistry (ICC)

See 1 publication below

Western Blot (WB)

See 5 publications below

Flow Cytometry (Flow)

See 1 publication below

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Rat, Zebrafish

Published species

Cat, Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2273801

Product Specific Information

PA1-047 detects alpha-1 adrenergic receptor (A1AR) from human, mouse, rat, zebra fish (Danio rerio) and Drosophila samples.

PA1-047 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing the A1AR from mouse kidney membrane preparations, mouse liver, HeLa cell lysates, zebra fish and drosophila samples. Immunofluorescence staining of A1AR in mouse kidney distal tubule cells with PA1-047 results in staining of the plasma membrane.

The PA1-047 immunogen is a synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR. This sequence is 100% conserved in all A1AR subtypes examined. PA1-047 immunizing peptide (Cat. # PEP-216) is available for use in neutralization and control experiments.

Target Information

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence.

A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ADA1; adrenergic alpha 1c receptor; adrenergic receptor alpha 1c subtype; adrenergic receptor, alpha 1a; adrenergic receptor, alpha 1c; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1Aa, receptor; adrenoceptor alpha 1A; alpha 1A-adrenoceptor; alpha 1A-adrenoreceptor; alpha 1C-adrenergic receptor; Alpha-1 adrenoceptor; Alpha-1 adrenoreceptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoceptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; G protein coupled receptor

Gene Aliases: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR; fc18h12; wu:fc18h12

UniProt ID: (Human) P35348, (Mouse) P97718, (Rat) P43140

Entrez Gene ID: (Human) 148, (Mouse) 11549, (Rat) 29412, (Zebrafish) 798498

Molecular Function: G-protein coupled receptor receptor