Western blot analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), T47D (Lane 3) and HEL 92.1.7 (lane 4). The blots were probed with Anti-alpha-2a Adrenergic Receptor Rabbit Polyclonal Antibody (Product# PA1048, 1:250-1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G21234,1:5000 dilution). Two bands 60 and 50 kDa corresponding to alpha-2a Adrenergic Receptor was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corressponding to residues R(218) I Y Q I A K R R T R V P P S R R G(235) of the 3rd intracellular loop of human A2AAR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1,000|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-048 detects alpha-2A adrenergic receptor (A2AAR) from human, rat and mouse tissues.
PA1-048 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~45 kDa protein representing A2AAR from mouse kidney membrane preparations.
The PA1-048 immunogen is a synthetic peptide corressponding to residues R(218) I Y Q I A K R R T R V P P S R R G(235) of the 3rd intracellular loop of human A2AAR. This sequence is completely conserved between human, mouse, rat, and porcine A2AAR.
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence.
A2AR subtypes inhibit adenylyl cyclase, suppress voltage-sensitive calcium channels, and activate receptor-dependent potassium channels. All of the A2AR subtypes inhibit adenylyl cyclase through coupling to members of the Gi/Go class of G proteins. They are an essential component of the neural circuitry regulating cardiovascular physiology. The physiological function of the A2ARs in the kidney is to regulate sodium/hydrogen exchange although the role of A2AR subtypes remains to be precisely determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Single-cell analysis of long non-coding RNAs in the developing human neocortex.
PA1-048 was used in immunohistochemistry - frozen section to study the developing human neocortex for single-cell analysis of long non-coding RNAs
|Liu SJ,Nowakowski TJ,Pollen AA,Lui JH,Horlbeck MA,Attenello FJ,He D,Weissman JS,Kriegstein AR,Diaz AA,Lim DA||Genome biology (17:null)||2016|
Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex.
PA1-048 was used in immunohistochemistry - frozen section to analyze developing cerebral cortex and low-coverage single-cell mRNA sequencing that reveals activated signaling pathways and cellular heterogeneity
|Pollen AA,Nowakowski TJ,Shuga J,Wang X,Leyrat AA,Lui JH,Li N,Szpankowski L,Fowler B,Chen P,Ramalingam N,Sun G,Thu M,Norris M,Lebofsky R,Toppani D,Kemp DW,Wong M,Clerkson B,Jones BN,Wu S,Knutsson L,Alvarado B,Wang J,Weaver LS,May AP,Jones RC,Unger MA,Kriegstein AR,West JA||Nature biotechnology (32:1053)||2014|
Immunohistochemical localization of ¿2-adrenergic receptors in the neonatal rat cochlea and the vestibular labyrinth.
PA1-048 was used in immunohistochemistry to study the immunohistochemical expression of the three alpha2-adrenergic receptor sub-types in the cochlea and vestibular labyrinth of neonatal rats
|Cai J,Li J,Mao Y,Bai X,Xu L,Wang H||Journal of molecular neuroscience : MN (51:1010)||2013|
Alpha2-adrenergic regulation of NO production alters postoperative intestinal smooth muscle dysfunction in rodents.
PA1-048 was used in immunohistochemistry to investigate the possible effects of alpha 2-adrenergic blockade with yohimbine on postsurgical intestinal smooth muscle dysfunction and NO production
|Kreiss C,Toegel S,Bauer AJ||American journal of physiology. Gastrointestinal and liver physiology (287:G658)||2004|
Stimulation of ¿(2A)-adrenoceptors promotes the maturation of dendritic spines in cultured neurons of the medial prefrontal cortex.
PA1-048 was used in immunocytochemistry to study the role of the alpha2A-adrenoceptors in the maturation of dendritic spines in the medial prefrontal cortex
|Ren WW,Liu Y,Li BM||Molecular and cellular neurosciences (49:205)||2012|
Altered prejunctional modulation of intestinal cholinergic and noradrenergic pathways by alpha2-adrenoceptors in the presence of experimental colitis.
PA1-048 was used in western blot to investigate the role of alpha2-adrenoceptors in intestinal inflammation
|Blandizzi C,Fornai M,Colucci R,Baschiera F,Barbara G,De Giorgio R,De Ponti F,Breschi MC,Del Tacca M||British journal of pharmacology (139:309)||2003|