Proteintech

alpha Actin Polyclonal Antibody

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alpha Actin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 23660-1-AP (alpha Actin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

alpha Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

alpha Actin Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

alpha Actin Antibody in Immunoprecipitation (IP) — IP result of anti-alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

Product Details

23660-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:8,000	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	alpha Actin Fusion Protein Ag19151 (1-377 aa encoded by BC012597) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.22 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MCDEDETTA LVCDNGSGLV KAGFAGDDAP RAVFPSIVGR PRHQGVMVGM GQKDSYVGDE AQSKRGILTL KYPIEHGIIT NWDDMEKIWH HTFYNELRVA PEEHPTLLTE APLNPKANRE KMTQIMFETF NVPAMYVAIQ AVLSLYASGR TTGIVLDSGD GVTHNVPIYE GYALPHAIMR LDLAGRDLTD YLMKILTERG YSFVTTAERE IVRDIKEKLC YVALDFENEM ATAASSSSLE KSYELPDGQV ITIGNERFRC PETLFQPSFI GMESAGIHET TYNSIMKCDI DIRKDLYANN VMSGGTTMYP GIADRMQKEI TALAPSTMKI KIIAPPERKY SVWIGGSILA SLSTFQQMWI TKQEYDEAGP SIVHRKCF (1-377 aa encoded by BC012597)

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Vinexin β Interacts with Hepatitis C Virus NS5A, Modulating Its Hyperphosphorylation To Regulate Viral Propagation.

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Bioinformatics

Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; alpha-actin cardiac; Alpha-actin-1; Alpha-cardiac actin; alphac-actin; nemaline myopathy type 3

Gene Aliases: AA959943; ACTA; Acta-2; ACTA1; ACTC; Actc-1; ACTC1; Acts; Actsk-1; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; LVNC4; MPFD; NEM1; NEM2; NEM3; SHPM

UniProt ID: (Human) P68133, (Human) P68032, (Mouse) P68134, (Rat) P68136, (Mouse) P68033, (Rat) P68035

Entrez Gene ID: (Human) 58, (Human) 70, (Mouse) 11459, (Rat) 29437, (Mouse) 11464, (Rat) 29275

Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein