|Tested species reactivity||Human, Rat|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||3 µg/ml|
|Immunofluorescence (IF)||3 ug/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-067 detects coatomer-protein I alpha (COPI alpha) from human and rat cells.
PA1-067 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing COP alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha-COP using PA1-067 results in alpha-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.
PA1-067 immunogen is a synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP. The PA1-067 immunizing peptide (Cat. # PEP-125) is available for use in neutralization and control experiments.
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Intracellular trafficking of KA2 kainate receptors mediated by interactions with coatomer protein complex I (COPI) and 14-3-3 chaperone systems.
PA1-067 was used in immunoprecipitation to the mechanism for the intracellular trafficking of KA2 kainate receptors .
|Vivithanaporn P,Yan S,Swanson GT||The Journal of biological chemistry (281:15475)||2006|
The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex.
PA1-067 was used in western blot to investigate the association between the human phosphatidylinositol phosphatase SAC1 and the coatomer I complex.
|Rohde HM,Cheong FY,Konrad G,Paiha K,Mayinger P,Boehmelt G||The Journal of biological chemistry (278:52689)||2003|