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AbboMax

bNOS Polyclonal Antibody

View all (39) nNOS antibodies

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Product Details

500-3824

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL
-

Immunoprecipitation (IP)

2-5 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corrsponding to the internal segment of human bNOS

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Human fetal brain lysate, liver, retina

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bNOS1; Brain NOS; constitutive NOS; N-NOS; NC-NOS; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; unnamed protein product

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Gene Aliases: bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS

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UniProt ID: (Human) P29475, (Rat) P29476

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Entrez Gene ID: (Human) 4842, (Rat) 24598

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Function(s)
nitric-oxide synthase activity calcium channel regulator activity protein binding calmodulin binding FMN binding oxidoreductase activity sodium channel regulator activity heme binding tetrahydrobiopterin binding arginine binding peptidyl-cysteine S-nitrosylase activity ion channel binding cadmium ion binding calcium-dependent protein binding flavin adenine dinucleotide binding NADP binding scaffold protein binding zinc ion binding enzyme binding identical protein binding ATPase binding phosphoprotein binding RNA polymerase II sequence-specific DNA binding transcription factor binding NADPH binding
Process(es)
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process striated muscle contraction cell communication myoblast fusion blood circulation response to heat response to hormone negative regulation of calcium ion transport into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of metal ion transport signaling response to lipopolysaccharide multicellular organismal response to stress nitric oxide-cGMP-mediated signaling pathway xenobiotic catabolic process vasodilation negative regulation of potassium ion transport negative regulation of ion transport positive regulation of neuron apoptotic process cell redox homeostasis negative regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of cardiac muscle contraction regulation of postsynaptic membrane potential cellular response to growth factor stimulus positive regulation of the force of heart contraction synaptic signaling by nitric oxide positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of blood circulation positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential system process female pregnancy negative regulation of cell proliferation response to mechanical stimulus response to lead ion response to activity peptidyl-cysteine S-nitrosylation response to nutrient levels response to vitamin E response to vitamin B3 negative regulation of iron ion transmembrane transport response to nicotine response to decreased oxygen levels regulation of apoptotic process negative regulation of apoptotic process response to peptide hormone negative regulation of neuron apoptotic process response to estrogen establishment of protein localization response to ethanol positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of vasoconstriction nitric oxide metabolic process negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of hepatic stellate cell contraction cellular response to mechanical stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of adrenergic receptor signaling pathway positive regulation of long-term synaptic potentiation
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