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Bioss
beta-Actin Polyclonal Antibody
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FIGURE: 1 / 4
beta-Actin Antibody (BS-0061R) in WB
Lane 1: Mouse lung lysates; Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Product Details
BS-0061R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
All,
Bovine,
Dog,
Goat,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
MAP conjugated synthetic peptide derived from human beta Actin, amino acids 1-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin, beta; Actin, cytoplasmic 1; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; cytoskeletal beta actin; gamma non-muscle actin; PS1TP5-binding protein 1
Gene Aliases: ACT-5; ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1
UniProt ID: (Sheep) P60713, (Rabbit) P29751, (Bovine) P60712, (Human) P60709, (Pig) Q6QAQ1, (Dog) O18840, (Rat) P60711, (Mouse) P60710
Entrez Gene ID: (Sheep) 443052, (Rabbit) 100009272, (Bovine) 280979, (Goat) 102179831, (Human) 60, (Pig) 414396, (Dog) 403580, (Rat) 81822, (Mouse) 11461
protein binding
ATP binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
nucleosomal DNA binding
protein kinase binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
retina development in camera-type eye
cellular response to electrical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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