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beta Adducin Polyclonal Antibody
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FIGURE: 1 / 1
beta Adducin Antibody (BS-1114R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with beta Adducin Polyclonal Antibody, Unconjugated ( bs-1114R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staini... View More
Product Details
BS-1114R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human beta Adducin protein, amino acids 401-500.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Add97; adducin 2 (beta); adducin beta; Adducin-63; Beta-adducin; Erythrocyte adducin subunit beta; snoRNA MBII-396; unnamed protein product
Gene Aliases: 2900072M03Rik; ADD2; add97; ADDB; beta-ADD
UniProt ID: (Human) P35612, (Mouse) Q9QYB8, (Rat) Q05764
Entrez Gene ID: (Human) 119, (Mouse) 11519, (Rat) 24171
actin binding
structural constituent of cytoskeleton
protein binding
calmodulin binding
cytoskeletal adaptor activity
protein kinase binding
spectrin binding
protein homodimerization activity
protein heterodimerization activity
protein dimerization activity
actin filament binding
structural molecule activity
non-motor actin binding protein
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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