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      beta Amyloid (1-40) Polyclonal Antibody

      See 45 other beta Amyloid antibodies ›
      beta Amyloid (1-40) Polyclonal Antibody for IHC, ELISA, RIA and DB

      Catalog#: 44-136 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      44-136 100 µg
      355.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
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      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human
      Published species reactivity Human, Mouse, Not Applicable
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen The antiserum was produced against a chemically synthesized peptide corresponding to amino acids 1-40 of human beta-Amyloid (A-beta).
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.5 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer Dulbecco's PBS, pH 7.3
      Contains no preservative
      Storage Conditions -80°C
      Tested Applications Dilution *
      Dot blot (DB) Assay Dependent
      ELISA (ELISA) 1µg/ml
      Immunohistochemistry (IHC) Assay Dependent
      Radioimmune Assays (RIA) Assay Dependent

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Western Blot (WB) See 4 publications below
      Immunohistochemistry (Frozen) (IHC (F)) See 1 publications below
      Immunofluorescence (IF) See 1 publications below
      Immunohistochemistry (IHC) See 1 publications below
      Immunohistochemistry - Free Floating (IHC (Free)) See 1 publications below
      Immunoprecipitation (IP) See 1 publications below
      Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) See 1 publications below
      ELISA (ELISA) See 1 publications below

      Product Specific Information

      No significant cross-reactivity to beta-Amyloid 42 has been observed. Previous lots of this antibody recognized the sequence of beta-Amyloid [1-40] in the region from amino acids 15-30. No blocking activity was observed with beta-Amyloid [1-12] whereas the peptides beta-Amyloid [14-35], beta-Amyloid [15-28], beta-Amyloid [17-30] and Beta-Amyloid [1-40] were all able to block antibody activity.

      Background/Target Information

      Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer and quote;s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of betaAPP and helps to modulate the production of the amyloid beta peptide.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited Histamine induces Egr-1 expression in human aortic endothelial cells via the H1 receptor-mediated protein kinase Cdelta-dependent ERK activation pathway. Hao F,Tan M,Xu X,Cui MZ The Journal of biological chemistry (283:26928) 2008
      Human Not Cited Efficient inhibition of beta-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes. Nawrot B,Antoszczyk S,Maszewska M,Kuwabara T,Warashina M,Taira K,Stec WJ European journal of biochemistry (270:3962) 2003
      Mouse Not Cited Apolipoprotein E4 influences amyloid deposition but not cell loss after traumatic brain injury in a mouse model of Alzheimer's disease. Hartman RE,Laurer H,Longhi L,Bales KR,Paul SM,McIntosh TK,Holtzman DM The Journal of neuroscience : the official journal of the Society for Neuroscience (22:10083) 2002
      Mouse Not Cited Human and murine ApoE markedly alters A beta metabolism before and after plaque formation in a mouse model of Alzheimer's disease. Fagan AM,Watson M,Parsadanian M,Bales KR,Paul SM,Holtzman DM Neurobiology of disease (9:305) 2002

      Immunohistochemistry (Frozen)

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable 1:2000 Selective contrast enhancement of individual Alzheimer's disease amyloid plaques using a polyamine and Gd-DOTA conjugated antibody fragment against fibrillar Abeta42 for magnetic resonance molecular imaging.
      44-136 was used in immunohistochemistry - frozen section to describe using the F(ab')2 fragment of a monoclonal antibody raised against fibrillar human Abeta42 as a diagnostic imaging probe for AD       		Ramakrishnan M,Wengenack TM,Kandimalla KK,Curran GL,Gilles EJ,Ramirez-Alvarado M,Lin J,Garwood M,Jack CR,Poduslo JF Pharmaceutical research (25:1861) 2008

      Immunofluorescence

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse
      		Not Cited
      		Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation. Oakley H,Cole SL,Logan S,Maus E,Shao P,Craft J,Guillozet-Bongaarts A,Ohno M,Disterhoft J,Van Eldik L,Berry R,Vassar R The Journal of neuroscience : the official journal of the Society for Neuroscience (26:10129) 2006

      Immunohistochemistry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse Not Cited Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation. Oakley H,Cole SL,Logan S,Maus E,Shao P,Craft J,Guillozet-Bongaarts A,Ohno M,Disterhoft J,Van Eldik L,Berry R,Vassar R The Journal of neuroscience : the official journal of the Society for Neuroscience (26:10129) 2006

      Immunohistochemistry - Free Floating

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable 1:10,000 Time-dependent reduction in Abeta levels after intracranial LPS administration in APP transgenic mice.
      44-136 was used in immunohistochemistry - free floating to report that activated brain glia rapidly and transiently clear diffuse Abeta deposits but not compacted fibrillar amyloid       		Herber DL,Roth LM,Wilson D,Wilson N,Mason JE,Morgan D,Gordon MN Experimental neurology (190:245) 2004

      Immunoprecipitation

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human
      		Not Cited
      		Efficient inhibition of beta-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes. Nawrot B,Antoszczyk S,Maszewska M,Kuwabara T,Warashina M,Taira K,Stec WJ European journal of biochemistry (270:3962) 2003

      Immunohistochemistry (Paraffin, Frozen)

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse Not Cited Passive immunization against beta-amyloid peptide protects central nervous system (CNS) neurons from increased vulnerability associated with an Alzheimer's disease-causing mutation. Mohajeri MH,Saini K,Schultz JG,Wollmer MA,Hock C,Nitsch RM The Journal of biological chemistry (277:33012) 2002

      ELISA

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human
      Mouse       		Not Cited
      Not Cited       		Plaque-associated disruption of CSF and plasma amyloid-beta (Abeta) equilibrium in a mouse model of Alzheimer's disease. DeMattos RB,Bales KR,Parsadanian M,O'Dell MA,Foss EM,Paul SM,Holtzman DM Journal of neurochemistry (81:229) 2002

      Bioinformatics

      Protein Aliases:

      AAA; ABETA; ABPP; AD1; Alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; Amyloid beta A4 protein; Amyloid precursor protein; Amyloidogenic glycoprotein AG; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); Beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Peptidase nexin-II; PN-II; PN2; PreA4; Protease nexin-II; testicular tissue protein Li 2

      Gene Aliases:

      A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

      UniProt ID:

      (Human) B4DII8

      Entrez Gene ID:

      (Human) 351

      Molecular Function:

      signaling molecule

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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