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beta Amyloid (1-40) Polyclonal Antibody

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beta Amyloid (1-40) Antibody — Amyloid Beta (Abeta) (N-terminal) Polyclonal Antibody, Rabbit

beta Amyloid (1-40) Antibody in Immunohistochemistry (IHC) — ijms-21-02295-sch001_Scheme A1 Scheme A1 Semi-automatic measurement of senile plaques in the regions of interest (cortex and the hippocampus). Upper line: Measurement of the area of the section: (1) Filling of the section ( a ) with red color ( b ); (2) dichotomization of color into black and white using a semiautomatic method with an appropriate color threshold ( c ). Lower line: Measurement of the total area of the senile plaque: (1) digital stripping of Abeta-stained lesions located out of the regions of interest ( d ); (2) change of color of the remaining Abeta-stained area to red using manual calibration with an appropriate color threshold ( e ); (3) dichotomization of color to black (Abeta-stained lesions in regions of interest) and white (other) using a semiautomatic method with an appropriate color threshold ( f ).

beta Amyloid (1-40) Antibody in Western Blot (WB) — Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Western Blot

beta Amyloid (1-40) Antibody in ELISA (ELISA) — Figure 4 Single intravenous injection of SST-scFv8D3 increases brain levels of neprilysin but has no detectable effect on Abeta levels. (A). Comparison of neprilysin expression in the brain of wild-type mice (n=4), eight-months old APPswe (n=7) and fourteen-months old APPswe mice (n=4). APPswe mice exhibit a significant reduction in neprilysin levels compared to wild-type. A significant age-dependent decline in neprilysin protein levels is detected between the APPswe groups. (B). Schematic representation of the first therapeutic study with SST-scFv8D3. Fourteen-month old transgenic mice harbouring the Swedish mutation in APP (APPswe) injected intravenously with 2 mg/kg of SST-scFv8D3 (n=4) or PBS (n=4) as a negative control. Mice euthanized 24 h post injection by perfusion with physiological saline. (C). Significant increase in the levels of neprilysin in the membrane fraction (TBS-Triton soluble) of the brain of the treatment group. No differences detected in the levels of Abeta40 and Abeta42, neither in the soluble pool (D), nor in the membrane fraction (E) between SST-scFv8D3 treated group and the controls. Results are presented as mean +-SD. One-way ANOVA followed by Tukey's post hoc test (A) and unpaired t-test (C-E) were applied to measure the presence of statistically significant differences in the results. A significant p-value is defined as: >0.5 (ns), <0.05 (*), <0.01 (**), <0.005 (***).

Product Details

44-136

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 5 publications 5 publications
Immunohistochemistry (IHC)	Assay-dependent	View 8 publications 8 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 3 publications 3 publications
Immunohistochemistry - Free Floating (IHC (Free))	-	View 1 publication 1 publication
ELISA (ELISA)	1 µg/mL	View 2 publications 2 publications
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Radioimmune Assays (RIA)	Assay-dependent	-
Dot blot (DB)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Non-human primate
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	The antiserum was produced against a chemically synthesized peptide corresponding to amino acids 1-40 of human beta-Amyloid (A-beta). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	-20°C
RRID	AB_2533599

Product Specific Information

No significant cross-reactivity to beta-Amyloid 42 has been observed. Previous lots of this antibody recognized the sequence of beta-Amyloid [1-40] in the region from amino acids 15-30. No blocking activity was observed with beta-Amyloid [1-12] whereas the peptides beta-Amyloid [14-35], beta-Amyloid [15-28], beta-Amyloid [17-30] and Beta-Amyloid [1-40] were all able to block antibody activity.

Target Information

Beta-amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhanced neprilysin-mediated degradation of hippocampal Aβ42 with a somatostatin peptide that enters the brain.

Theranostics

Rofo F,Ugur Yilmaz C,Metzendorf N,Gustavsson T,Beretta C,Erlandsson A,Sehlin D,Syvänen S,Nilsson P,Hultqvist G

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in ELISA

Wed Aug 04 00:00:00 EDT 2021

Evidence of Tau Hyperphosphorylation and Dystrophic Microglia in the Common Marmoset.

Frontiers in aging neuroscience

Rodriguez-Callejas JD,Fuchs E,Perez-Cruz C

44136 was used in immunohistochemistry - frozen section to evaluate cellular markers associated with neurodegenerative diseases in common marmoset (Callithrix jacchus) brains

Thu Oct 01 00:00:00 EDT 2020

Low-Dose Phosphodiesterase III Inhibitor Reduces the Vascular Amyloid Burden in Amyloid-β Protein Precursor Transgenic Mice.

International journal of molecular sciences

Yakushiji Y,Kawamoto K,Uchihashi K,Ihara M,Aoki S,Nagaishi Y,Suzuyama K,Tsugitomi Y,Hara H

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Immunohistochemistry

Thu Mar 26 00:00:00 EDT 2020

Low-Dose Phosphodiesterase III Inhibitor Reduces the Vascular Amyloid Burden in Amyloid-β Protein Precursor Transgenic Mice.

International journal of molecular sciences

Yakushiji Y,Kawamoto K,Uchihashi K,Ihara M,Aoki S,Nagaishi Y,Suzuyama K,Tsugitomi Y,Hara H

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Immunohistochemistry

Thu Mar 26 00:00:00 EDT 2020

Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice.

Antioxidants (Basel, Switzerland)

Matthews DG,Caruso M,Murchison CF,Zhu JY,Wright KM,Harris CJ,Gray NE,Quinn JF,Soumyanath A

44-136 was used in Immunohistochemistry to study the effect of Centella asiatica water extract treatment on oxidative stress in Alzheimer's disease model mice.

Sun Dec 08 00:00:00 EST 2019

Effects of rising amyloidβ levels on hippocampal synaptic transmission, microglial response and cognition in APP_Swe/PSEN1_M146V transgenic mice.

EBioMedicine

Medawar E,Benway TA,Liu W,Hanan TA,Haslehurst P,James OT,Yap K,Muessig L,Moroni F,Nahaboo Solim MA,Baidildinova G,Wang R,Richardson JC,Cacucci F,Salih DA,Cummings DM,Edwards FA

44-136 was used in Immunohistochemistry-immunofluorescence to investigate CA1 synaptic transmission and plasticity.

Tue Jan 01 00:00:00 EST 2019

Corticotropin-releasing factor overexpression gives rise to sex differences in Alzheimer's disease-related signaling.

Molecular psychiatry

Bangasser DA,Dong H,Carroll J,Plona Z,Ding H,Rodriguez L,McKennan C,Csernansky JG,Seeholzer SH,Valentino RJ

44136 was used in immunohistochemistry to discuss the role of corticotropin-releasing factor in the sex bias of some neuropsychiatric and neurodegenerative disorders

Tue Aug 01 00:00:00 EDT 2017

Amyloid-β oligomerization in Alzheimer dementia versus high-pathology controls.

Annals of neurology

Esparza TJ,Zhao H,Cirrito JR,Cairns NJ,Bateman RJ,Holtzman DM,Brody DL

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Western Blot

Tue Jan 01 00:00:00 EST 2013

Amyloid-β oligomerization in Alzheimer dementia versus high-pathology controls.

Annals of neurology

Esparza TJ,Zhao H,Cirrito JR,Cairns NJ,Bateman RJ,Holtzman DM,Brody DL

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Western Blot

Tue Jan 01 00:00:00 EST 2013

Suppressed accumulation of cerebral amyloid {beta} peptides in aged transgenic Alzheimer's disease mice by transplantation with wild-type or prostaglandin E2 receptor subtype 2-null bone marrow.

The American journal of pathology

Keene CD,Chang RC,Lopez-Yglesias AH,Shalloway BR,Sokal I,Li X,Reed PJ,Keene LM,Montine KS,Breyer RM,Rockhill JK,Montine TJ

Published figure using beta Amyloid (1-40) polyclonal antibody (Product # 44-136) in Immunofluorescence

Thu Jul 01 00:00:00 EDT 2010

SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells.

Nature cell biology

Bell RD,Deane R,Chow N,Long X,Sagare A,Singh I,Streb JW,Guo H,Rubio A,Van Nostrand W,Miano JM,Zlokovic BV

44-136 was used in Immunofluorescence in immunohistochemistry on frozen tissues to suggest that serum response factor and myocardin function as a transcriptional switch, controlling Abeta cerebrovascular clearance and Alzheimer's disease progression.

Sun Feb 01 00:00:00 EST 2009

SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells.

Nature cell biology

Bell RD,Deane R,Chow N,Long X,Sagare A,Singh I,Streb JW,Guo H,Rubio A,Van Nostrand W,Miano JM,Zlokovic BV

Sun Feb 01 00:00:00 EST 2009

Histamine induces Egr-1 expression in human aortic endothelial cells via the H1 receptor-mediated protein kinase Cdelta-dependent ERK activation pathway.

The Journal of biological chemistry

Hao F,Tan M,Xu X,Cui MZ

Fri Oct 03 00:00:00 EDT 2008

Selective contrast enhancement of individual Alzheimer's disease amyloid plaques using a polyamine and Gd-DOTA conjugated antibody fragment against fibrillar Abeta42 for magnetic resonance molecular imaging.

Pharmaceutical research

Ramakrishnan M,Wengenack TM,Kandimalla KK,Curran GL,Gilles EJ,Ramirez-Alvarado M,Lin J,Garwood M,Jack CR,Poduslo JF

44-136 was used in immunohistochemistry - frozen section to describe using the F(ab')2 fragment of a monoclonal antibody raised against fibrillar human Abeta42 as a diagnostic imaging probe for AD

Fri Aug 01 00:00:00 EDT 2008

Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Oakley H,Cole SL,Logan S,Maus E,Shao P,Craft J,Guillozet-Bongaarts A,Ohno M,Disterhoft J,Van Eldik L,Berry R,Vassar R

Wed Oct 04 00:00:00 EDT 2006

Time-dependent reduction in Abeta levels after intracranial LPS administration in APP transgenic mice.

Experimental neurology

Herber DL,Roth LM,Wilson D,Wilson N,Mason JE,Morgan D,Gordon MN

44-136 was used in immunohistochemistry - free floating to report that activated brain glia rapidly and transiently clear diffuse Abeta deposits but not compacted fibrillar amyloid

Mon Nov 01 00:00:00 EST 2004

Efficient inhibition of beta-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes.

European journal of biochemistry

Nawrot B,Antoszczyk S,Maszewska M,Kuwabara T,Warashina M,Taira K,Stec WJ

Wed Oct 01 00:00:00 EDT 2003

Efficient inhibition of beta-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes.

European journal of biochemistry

Nawrot B,Antoszczyk S,Maszewska M,Kuwabara T,Warashina M,Taira K,Stec WJ

Wed Oct 01 00:00:00 EDT 2003

Apolipoprotein E4 influences amyloid deposition but not cell loss after traumatic brain injury in a mouse model of Alzheimer's disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hartman RE,Laurer H,Longhi L,Bales KR,Paul SM,McIntosh TK,Holtzman DM

Sun Dec 01 00:00:00 EST 2002

Passive immunization against beta-amyloid peptide protects central nervous system (CNS) neurons from increased vulnerability associated with an Alzheimer's disease-causing mutation.

The Journal of biological chemistry

Mohajeri MH,Saini K,Schultz JG,Wollmer MA,Hock C,Nitsch RM

Fri Sep 06 00:00:00 EDT 2002

Plaque-associated disruption of CSF and plasma amyloid-beta (Abeta) equilibrium in a mouse model of Alzheimer's disease.

Journal of neurochemistry

DeMattos RB,Bales KR,Parsadanian M,O'Dell MA,Foss EM,Paul SM,Holtzman DM

Mon Apr 01 00:00:00 EST 2002

Plaque-associated disruption of CSF and plasma amyloid-beta (Abeta) equilibrium in a mouse model of Alzheimer's disease.

Journal of neurochemistry

DeMattos RB,Bales KR,Parsadanian M,O'Dell MA,Foss EM,Paul SM,Holtzman DM

Mon Apr 01 00:00:00 EST 2002

Human and murine ApoE markedly alters A beta metabolism before and after plaque formation in a mouse model of Alzheimer's disease.

Neurobiology of disease

Fagan AM,Watson M,Parsadanian M,Bales KR,Paul SM,Holtzman DM

Mon Apr 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: Abeta40; Abeta42; ABPP; AICD-50; AICD-57; AICD-59; Alpha-CTF; Alpha-secretase C-terminal fragment; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid precursor protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Beta-APP40; Beta-APP42; Beta-CTF; Beta-secretase C-terminal fragment; C31; C80; C83; C99; Cerebral vascular amyloid peptide; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Gamma-secretase C-terminal fragment 50; Gamma-secretase C-terminal fragment 57; Gamma-secretase C-terminal fragment 59; N-APP; P3(40); P3(42); peptidase nexin-II; PN-II; PreA4; Protease nexin-II; S-APP-alpha; S-APP-beta; Soluble APP-alpha; Soluble APP-beta; testicular tissue protein Li 2

Gene Aliases: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

UniProt ID: (Human) P05067

Entrez Gene ID: (Human) 351

Molecular Function: protease inhibitor