|Dot blot (DB)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized peptide corresponding to amino acids 1-40 of human beta-Amyloid (A-beta).|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3|
No significant cross-reactivity to beta-Amyloid 42 has been observed. Previous lots of this antibody recognized the sequence of beta-Amyloid [1-40] in the region from amino acids 15-30. No blocking activity was observed with beta-Amyloid [1-12] whereas the peptides beta-Amyloid [14-35], beta-Amyloid [15-28], beta-Amyloid [17-30] and Beta-Amyloid [1-40] were all able to block antibody activity.
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer and quote;s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of betaAPP and helps to modulate the production of the amyloid beta peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AAA; ABETA; ABPP; AD1; Alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; Amyloid beta A4 protein; Amyloid precursor protein; Amyloidogenic glycoprotein AG; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); Beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Peptidase nexin-II; PN-II; PN2; PreA4; Protease nexin-II; testicular tissue protein Li 2
Gene Aliases: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2
UniProt ID: (Human) B4DII8
Entrez Gene ID: (Human) 351
Molecular Function: signaling molecule
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.